<\/code><\/pre>\n\n\n\n\n\nLOCUS QOS45029 1273 aa linear SYN 28-OCT-2020\r\nDEFINITION SARS-CoV-2 spike [synthetic construct].\r\nACCESSION QOS45029\r\nVERSION QOS45029.1\r\nDBSOURCE accession MW036243.1<\/pre>\nKEYWORDS .\r\nSOURCE synthetic construct\r\n ORGANISM synthetic construct<\/pre>\n other sequences; artificial sequences.\r\nREFERENCE 1 (residues 1 to 1273)\r\n AUTHORS Wussow,F., Chiuppesi,F. and Diamond,D.\r\n TITLE Development of a Multi-Antigenic SARS-CoV-2 Vaccine Using a\r\n Synthetic Poxvirus Platform\r\n JOURNAL Unpublished\r\nREFERENCE 2 (residues 1 to 1273)\r\n AUTHORS Wussow,F., Chiuppesi,F. and Diamond,D.\r\n TITLE Direct Submission\r\n JOURNAL Submitted (23-SEP-2020) Hematology, City of Hope, 1500 E Duarte Rd,\r\n Duarte, CA 91010, USA\r\nFEATURES Location\/Qualifiers\r\n source 1..1273\r\n \/organism=\"synthetic construct\"\r\n \/db_xref=\"taxon:32630<\/a>\"\r\n \/clone=\"C35\/41\"\r\n<\/span> Protein<\/a> 1..1273\r\n \/product=\"SARS-CoV-2 spike\"\r\n<\/span> CDS<\/a> 1..1273\r\n \/coded_by=\"MW036243.1:145058..148879\"\r\n \/transl_table=11<\/a>\r\n<\/span>ORIGIN \r\n 1 mfvflvllpl vssqcvnltt rtqlppaytn sftrgvyypd kvfrssvlhs tqdlflpffs<\/span>\r\n 61 nvtwfhaihv sgtngtkrfd npvlpfndgv yfasteksni irgwifgttl dsktqslliv<\/span>\r\n 121 nnatnvvikv cefqfcndpf lgvyyhknnk swmesefrvy ssannctfey vsqpflmdle<\/span>\r\n 181 gkqgnfknlr efvfknidgy fkiyskhtpi nlvrdlpqgf saleplvdlp iginitrfqt<\/span>\r\n 241 llalhrsylt pgdsssgwta gaaayyvgyl qprtfllkyn engtitdavd caldplsetk<\/span>\r\n 301 ctlksftvek giyqtsnfrv qptesivrfp nitnlcpfge vfnatrfasv yawnrkrisn<\/span>\r\n 361 cvadysvlyn sasfstfkcy gvsptklndl cftnvyadsf virgdevrqi apgqtgkiad<\/span>\r\n 421 ynyklpddft gcviawnsnn ldskvggnyn ylyrlfrksn lkpferdist eiyqagstpc<\/span>\r\n 481 ngvE<\/strong>gfncyf plqsygfqpt N<\/strong>gvgyqpyrv vvlsfellha patvcgpkks tnlvknkcvn<\/span>\r\n 541 fnfngltgtg vltesnkkfl pfqqfgrdia dttdavrdpq tleilditpc sfggvsvitp<\/span>\r\n 601 gtntsnqvav lyqdvnctev pvaihadqlt ptwrvystgs nvfqtragcl igaehvnnsy<\/span>\r\n 661 ecdipigagi casyqtqtns prrarsvasq siiaytmslg aensvaysnn siaiptnfti<\/span>\r\n 721 svtteilpvs mtktsvdctm yicgdstecs nlllqygsfc tqlnraltgi aveqdkntqe<\/span>\r\n 781 vfaqvkqiyk tppikdfggf nfsqilpdps kpskrsfied llfnkvtlad agfikqygdc<\/span>\r\n 841 lgdiaardli caqkfngltv lpplltdemi aqytsallag titsgwtfga gaalqipfam<\/span>\r\n 901 qmayrfngig vtqnvlyenq klianqfnsa igkiqdslss tasalgklqd vvnqnaqaln<\/span>\r\n 961 tlvkqlssnf gaissvlndi lsrldkveae vqidrlitgr lqslqtyvtq qliraaeira<\/span>\r\n 1021 sanlaatkms ecvlgqskrv dfcgkgyhlm sfpqsaphgv vflhvtyvpa qeknfttapa<\/span>\r\n 1081 ichdgkahfp regvfvsngt hwfvtqrnfy epqiittdnt fvsgncdvvi givnntvydp<\/span>\r\n 1141 lqpeldsfke eldkyfknht spdvdlgdis ginasvvniq keidrlneva knlneslidl<\/span>\r\n 1201 qelgkyeqyi kwpwyiwlgf iagliaivmv timlccmtsc csclkgccsc gscckfdedd<\/span>\r\n 1261 sepvlkgvkl hyt<\/span>\r\n\/\/<\/pre>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\u611f\u67d3\u30fb\u4f1d\u64ad\u6027\u306e\u5897\u52a0\u304c\u61f8\u5ff5\u3055\u308c\u308bSARS-CoV-2\u306e\u5909\u7570 E484K N501Y<\/span><\/h2>\n\u591a\u304f\u306e\u5909\u7570\u304c\u767a\u751f\u3059\u308b\u306f\u305a\u306e\u30a6\u30a4\u30eb\u30b9\u3067\u3001E484K\u3084N501Y\u306e\u5909\u7570\u306f\u611f\u67d3\u6027\u3084\u4f1d\u64ad\u6027\u306e\u5897\u52a0\u304c\u61f8\u5ff5<\/a>\u3055\u308c\u308b\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u5909\u7570\u306e\u3042\u308b\u5834\u6240\u306f\u3001\u30d2\u30c8\u306e\u7d30\u80de\u306b\u4fb5\u5165\u3059\u308b\u969b\u306b\u307e\u305a\u7d50\u5408\u3059\u308b\u5206\u5b50\u3067\u3042\u308bangiotensin-converting enzyme2 (ACE2)\u3068\u306e\u7d50\u5408\u306b\u91cd\u8981\u3060\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3042\u305f\u308a\u3092\u6628\u5e74\u3082\u898b\u305f<\/a>\u8a18\u61b6\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u5909\u7570\u306e\u30a6\u30a4\u30eb\u30b9\u304c\u56fd\u5185\u3067\u3082\u5831\u544a\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u3066\u304d\u307e\u3057\u305f\u306e\u3067\u3001\u305d\u306e\u5909\u7570\u306e\u8d77\u3053\u308b\u90e8\u5206\u304c\u7acb\u4f53\u7684\u306b\u3069\u3046\u3044\u3046\u4f4d\u7f6e\u306b\u3042\u305f\u308b\u306e\u304b\u3001SARS-CoV-2 Spike\u3068AEC2\u304c\u7d50\u5408\u3057\u3066\u308b\u7acb\u4f53\u69cb\u9020\u3092\u8abf\u3079\u305f\u30c7\u30fc\u30bf<\/a>\u306e\u516c\u958b\u30c7\u30fc\u30bf\u3092\u5143\u306b\u898b\u3066\u307f\u307e\u3057\u305f\u3002\u307e\u305a\u306f\u91ce\u751f\u578b\u306eSpike\u3067\u793a\u3057\u307e\u3059\u3002\u7dd1\u8272\u306e\u30e2\u30b3\u30e2\u30b3\u3057\u305f\u306e\u304c\u5909\u7570\u3059\u308b\u3068\u60aa\u3044\u6027\u8cea\u3092\u7372\u5f97\u3059\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u5fc3\u914d\u3055\u308c\u3066\u3044\u308b\u5834\u6240\u306e\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3092\u793a\u3057\u307e\u3059\u3002\uff08\u4e0b\u56f3\uff09<\/p>\n![\"\"](\"https:\/\/plaza.umin.ac.jp\/~OIO\/wp-content\/uploads\/2021\/05\/Wildtype.jpg\")
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\u4e0a\u56f3\u306e\u5f53\u8a72\u306e\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3092\u3001\u5909\u7570\u3057\u305f\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306b\u7f6e\u304d\u63db\u3048\u305f\u56f3\u3082\u3057\u3081\u3057\u307e\u3059\u3002\uff08\u4e0b\u56f3\uff09\u5074\u9396\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u3060\u3051\u5c0f\u3055\u304f\u306a\u308b\u3088\u3046\u306b\u8abf\u6574\u306f\u3057\u307e\u3057\u305f\u304c\u3001\u793a\u3055\u308c\u305f\u69cb\u9020\u5168\u4f53\u306f\u52d5\u304b\u306a\u3044\u8a2d\u5b9a\u3067\u3059\u306e\u3067\u672c\u5f53\u306b\u3053\u306e\u5f62\u306b\u306a\u3063\u3066\u3044\u308b\u3068\u306f\u671f\u5f85\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n
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E484K\u306f\u8377\u96fb\u304c\u53cd\u8ee2\u3059\u308b<\/h2>\n
\u30a2\u30df\u30ce\u9178\u306e\u57fa\u672c\u7684\u306a\u5206\u985e\u306b\u8377\u96fb\u306e\u967d\u6027\u30fb\u9670\u6027\u304c\u3042\u308a\u307e\u3059\u3002E484K\u306f484\u756a\u76ee\u306eE\u304cK\u306b\u5909\u7570\u3057\u305f\u4e8b\u3092\u793a\u3057\u307e\u3059\u3002\u91ce\u751f\u578b\u306eE\u306f\u9670\u6027\u8377\u96fb\u3092\u793a\u3059\u9178\u6027\u6975\u6027\u5074\u9396\u30a2\u30df\u30ce\u9178\u3067\u3001\u4e00\u65b9\u5909\u7570\u578b\u306eK\u306f\u967d\u6027\u8377\u96fb\u3092\u793a\u3059\u5869\u57fa\u6027\u30a2\u30df\u30ce\u9178\u3067\u3059\u3002\u611f\u67d3\u6027\u7b49\u304c\u5897\u52a0\u3059\u308b\u61f8\u5ff5\u306f\u3001\u8377\u96fb\u304c\u9006\u8ee2\u3059\u308b\u3053\u3068\u3067ACE2\u3078\u306e\u7d50\u5408\u306b\u5909\u5316\u304c\u8d77\u304d\u308b\u3068\u63a8\u5b9a\u3055\u308c\u308b\u3053\u3068\u304b\u3089\u6765\u3066\u3044\u308b\u3082\u306e\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u30ef\u30af\u30c1\u30f3\u306e\u52b9\u679c\u304c\u6e1b\u5f31\u3059\u308b\u3068\u3044\u3046\u61f8\u5ff5\u3082\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u61f8\u5ff5\u306f\u3001\u30ef\u30af\u30c1\u30f3\u306e\u52b9\u679c\u304c\u7d50\u5408\u9818\u57df\uff08328-533aa\uff09\u306b\u5bfe\u3059\u308b\u6297\u4f53\u306b\u3088\u308b\u3068\u3053\u308d\u304c\u3042\u3063\u3066\u3001\u3053\u306e\u5909\u7570\u304c\u7d50\u5408\u9818\u57df\u306e\u4e2d\u5fc3\u4ed8\u8fd1\u306e484aa\u306b\u5b58\u5728\u3057\u3066\u3044\u308b\u70b9\u306b\u52a0\u3048\u3066\u3001\u8377\u96fb\u304c\u9006\u8ee2\u3059\u308b\u3053\u3068\u3067\u6297\u4f53\u306e\u7d50\u5408\u306b\u3082\u5909\u5316\u304c\u8d77\u304d\u308b\u3068\u63a8\u5b9a\u3055\u308c\u308b\u3068\u3053\u308d\u304b\u3089\u304d\u3066\u3044\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n
\u3061\u306a\u307f\u306b\u57fa\u790e\u77e5\u8b58\u3068\u3057\u3066\u30a2\u30df\u30ce\u9178\u5074\u9396\u306e\u6027\u8cea\u3068\u3001\u7565\u53f7\u3092\u307e\u3068\u3081\u305f\u7269\u3092\u30b3\u30fc\u30eb\u30c9\u30b9\u30d7\u30ea\u30f3\u30b0\u30cf\u30fc\u30d0\u30fc\uff08CSH\uff09\u306e\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u96c6\u306e\u3088\u3046\u306a\u66f8\u7c4d\u306eMolecula Cloning a laboratory manual volume 3\u304b\u3089\u629c\u7c8b\u3067\u3059\u3002E (Glu)\u304cAcidic group\u3067\u3001K(Lys)\u304cBasic group\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n
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SARS-CoV-2\uff08\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\uff09\u306eSpike\u30bf\u30f3\u30d1\u30af\u8cea Spike\u306e\u30a2\u30df\u30ce\u9178\u914d\u5217 \u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\u80ba\u708eCOVID-19\u306e\u539f\u56e0\u30a6\u30a4\u30eb\u30b9\u3067\u3042\u308b\u3001SARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u306eSpike\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u3064…<\/p>\n","protected":false},"author":1,"featured_media":2617,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-2613","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science"],"jetpack_featured_media_url":"https:\/\/plaza.umin.ac.jp\/~OIO\/wp-content\/uploads\/2021\/05\/wild-surface.jpg","jetpack_shortlink":"https:\/\/wp.me\/p9b6zl-G9","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=\/wp\/v2\/posts\/2613","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2613"}],"version-history":[{"count":0,"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=\/wp\/v2\/posts\/2613\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=\/wp\/v2\/media\/2617"}],"wp:attachment":[{"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2613"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2613"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/~OIO\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2613"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}