{"id":892,"date":"2025-03-21T12:02:36","date_gmt":"2025-03-21T03:02:36","guid":{"rendered":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/?page_id=892"},"modified":"2025-04-01T10:46:01","modified_gmt":"2025-04-01T01:46:01","slug":"info2-experiment","status":"publish","type":"page","link":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/info-2\/info2-experiment\/","title":{"rendered":"\u7dda\u866b\u3067\u3067\u304d\u308b\u304a\u3059\u3059\u3081\u5b9f\u9a13"},"content":{"rendered":"\n<p><em>C. elegans<\/em>\u3092\u4f7f\u3046\u3053\u3068\u3067\u3001\u907a\u4f1d\u5b50\u306e\u5909\u7570\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u7570\u5e38\u30fb\u8868\u73fe\u578b\u304c\u751f\u3058\u308b\u3068\u3044\u3046\u3053\u3068\u3092\u76f4\u63a5\u89b3\u5bdf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u304c\u751f\u7269\u306e\u5f62\u614b\u3084\u884c\u52d5\u306b\u3069\u306e\u3088\u3046\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u304b\u3092\u7406\u89e3\u3059\u308b\u624b\u304c\u304b\u308a\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<div id=\"toc_container\" class=\"wp-block-group pada_30\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">\u76ee\u6b21<\/h2>\n\n\n\n<ul class=\"wp-block-list list_none has-medium-font-size\">\n<li><strong><a href=\"#info2-midashi01\" data-type=\"internal\" data-id=\"#section-1\"><strong>\u884c\u52d5\u89b3\u5bdf\uff08\u521d\u5fc3\u8005\u5411\u3051\uff09<\/strong><\/a><\/strong><\/li>\n\n\n\n<li><strong><a href=\"#section-2\"><strong>\u5f62\u614b\u89b3\u5bdf<\/strong><\/a><\/strong><\/li>\n\n\n\n<li><strong><a href=\"#section-3\"><strong>\u4ea4\u914d\u5b9f\u9a13<\/strong><\/a><\/strong><\/li>\n\n\n\n<li><strong><a href=\"#section-4\"><strong>\u884c\u52d5\u5b9f\u9a13\uff1a\u7dda\u866b\u306e\u597d\u7269\u3092\u63a2\u3057\u3066\u307f\u3088\u3046<\/strong><\/a><\/strong><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div id=\"info2-midashi01\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">1.<strong>&nbsp; <strong>\u884c\u52d5\u89b3\u5bdf\uff08\u521d\u5fc3\u8005\u5411\u3051\uff09<\/strong><\/strong><\/h2>\n\n\n\n<p><em>C. elegans<\/em>\u306f\u30b7\u30e3\u30fc\u30ec\u306e\u4e0a\u3067S\u5b57\u3092\u63cf\u304d\u306a\u304c\u3089\u524d\u9032\u3057\u3001\u6642\u6298\u5f8c\u9000\u3059\u308b\u3068\u3044\u3046\u72ec\u7279\u306e\u52d5\u304d\u3092\u898b\u305b\u307e\u3059\u3002\u3053\u306e\u52d5\u304d\u306f\u3001\u904b\u52d5\u795e\u7d4c\u304c\u80cc\u5074\u3068\u8179\u5074\u306e\u7b4b\u8089\u3092\u4ea4\u4e92\u306b\u53ce\u7e2e\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u5236\u5fa1\u3055\u308c\u3066\u3044\u307e\u3059\u3002<em>unc-13<\/em>\u3068\u3044\u3046\u907a\u4f1d\u5b50\u306e\u5909\u7570\u4f53\u3092\u300c\u30ca\u30b7\u30e7\u30ca\u30eb\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9 (NBRP)\u7dda\u866b\u300d\u306e\u30b9\u30c8\u30c3\u30af\u30bb\u30f3\u30bf\u30fc\u304b\u3089\u5165\u624b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u5909\u7570\u4f53\u7dda\u866b\u3067\u306f\u795e\u7d4c\u304b\u3089\u7b4b\u8089\u306b\u4fe1\u53f7\u3092\u4f1d\u9054\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u5206\u5b50\u3092\u30b3\u30fc\u30c9\u3059\u308b\u907a\u4f1d\u5b50\u306b\u5909\u7570\u304c\u3042\u308b\u305f\u3081\u3001\u7b4b\u8089\u3092\u3046\u307e\u304f\u52d5\u304b\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3053\u306e\u5909\u7570\u306b\u3088\u3063\u3066\u3001<em>C. elegans<\/em>\u306e\u52d5\u304d\u306f\u3069\u306e\u3088\u3046\u306b\u5909\u5316\u3059\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u5b9f\u4f53\u9855\u5fae\u93e1\u3092\u7528\u3044\u3066\u91ce\u751f\u578b\u3068\u5909\u7570\u4f53\u306e\u6210\u4f53\u3067\u52d5\u304d\u306e\u9055\u3044\u3092\u89b3\u5bdf\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<p><em>unc-13<\/em>\u5909\u7570\u4f53:  <a href=\"https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm5530\">https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm5530<\/a><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n\n\n\n<div id=\"info2-midashi02\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">2.<strong>&nbsp; <strong>\u5f62\u614b\u89b3\u5bdf<\/strong><\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">2-1. \u5f62\u614b\u89b3\u5bdf-1 (\u521d\u7d1a\u8005~\u4e2d\u7d1a\u8005\u5411\u3051)<\/h3>\n\n\n\n<p>\u52d5\u304d\u3060\u3051\u3067\u306f\u306a\u304f\u3001\u4f53\u306e\u5927\u304d\u3055\u3084\u5f62\u72b6\u3082\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u5f71\u97ff\u3092\u53d7\u3051\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u3001<em>dpy-1<\/em>\u5909\u7570\u4f53\u306f\u4f53\u304c\u592a\u304f\u77ed\u304f\u306a\u308a\u3001<em>lon-1<\/em>\u5909\u7570\u4f53\u306f\u9006\u306b\u4f53\u304c\u7d30\u9577\u304f\u306a\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u5f62\u614b\u7684\u306a\u5909\u5316\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u3067\u3001\u907a\u4f1d\u5b50\u304c\u4f53\u306e\u69cb\u9020\u306b\u4e0e\u3048\u308b\u5f71\u97ff\u3092\u8996\u899a\u7684\u306b\u7406\u89e3\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002NBRP\u304b\u3089\u3001\u91ce\u751f\u578b\u306e\u7dda\u866b\u3068<em>dpy-1<\/em>\u3001<em>lon-1<\/em>\u5909\u7570\u4f53\u3092\u53d6\u308a\u5bc4\u305b\u3001\u5b9f\u4f53\u9855\u5fae\u93e1\u3092\u7528\u3044\u3066\u540c\u3058\u767a\u751f\u30b9\u30c6\u30fc\u30b8\u306e\u500b\u4f53\u306e\u4f53\u306e\u5f62\u306e\u9055\u3044\u3092\u6bd4\u8f03\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<p><em>dpy-1<\/em>\u5909\u7570\u4f53: <a href=\"https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm11382\">https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm11382<\/a><br><em>lon-1<\/em>\u5909\u7570\u4f53:  <a href=\"https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm12597\">https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm12597<\/a><\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">2-2. \u5f62\u614b\u89b3\u5bdf-2 (\u4e0a\u7d1a\u8005\u5411\u3051)<\/h3>\n\n\n\n<p><em>C. elegans<\/em>\u306e\u4f53\u5185\u3092\u8a73\u7d30\u306b\u89b3\u5bdf\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<em>C. elegans<\/em>\u306f\u900f\u660e\u306a\u4f53\u3092\u6301\u3061\u3001\u5185\u90e8\u306e\u69cb\u9020\u304c\u89b3\u5bdf\u3057\u3084\u3059\u304f\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u5b9f\u4f53\u9855\u5fae\u93e1\u3092\u7528\u3044\u3066\u3001\u8178\uff08\u6d88\u5316\u7ba1\uff09\u306e\u69cb\u9020\u3092\u78ba\u8a8d\u3057\u3001\u5909\u7570\u4f53\u3068\u91ce\u751f\u578b\u3067\u5f62\u614b\u7684\u306a\u9055\u3044\u304c\u3042\u308b\u304b\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<em>tat-1<\/em>\u5909\u7570\u4f53\u7dda\u866b\u3067\u306f\u3001\u7d30\u80de\u5185\u3067\u5c0f\u80de\u3092\u904b\u3076\u6a5f\u80fd\u306b\u7570\u5e38\u304c\u3042\u308a\u3001\u8178\u306e\u9846\u7c92\u304c\u5927\u304d\u304f\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u5909\u7570\u306b\u3088\u3063\u3066<em>C. elegans<\/em>\u306e\u8178\u306e\u898b\u3048\u65b9\u306f\u3069\u306e\u3088\u3046\u306b\u5909\u5316\u3059\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u5b9f\u4f53\u9855\u5fae\u93e1\u3092\u62e1\u5927\u3057\u3066\u3001\u91ce\u751f\u578b\u3068\u5909\u7570\u4f53\u306e\u6210\u4f53\u3067\u8178\u304c\u3069\u306e\u3088\u3046\u306b\u898b\u3048\u308b\u304b\u9055\u3044\u3092\u89b3\u5bdf\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<p><em>tat-1<\/em>\u5909\u7570\u4f53:   <a href=\"https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm3117\">https:\/\/shigen.nig.ac.jp\/c.elegans\/DetailsSearch?allele=tm3117<\/a><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n\n\n\n<div id=\"info2-midashi03\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">3. \u4ea4\u914d\u5b9f\u9a13<\/h2>\n\n\n\n<p>\u7dda\u866b<em>Caenorhabditis elegans<\/em>\u306b\u306f\u3001\u96cc\u96c4\u540c\u4f53\u3068\u30aa\u30b9\u306e2\u7a2e\u985e\u306e\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30aa\u30b9\u3068\u96cc\u96c4\u540c\u4f53\u3092\u639b\u3051\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u4f53\u3092\u4f5c\u6210\u3057\u305f\u308a\u3001\u8907\u6570\u306e\u5909\u7570\u3092\u6301\u3064\u7cfb\u7d71\u3092\u4f5c\u6210\u3057\u305f\u308a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">3-1. \u30aa\u30b9\u3092\u51fa\u3057\u3066\u307f\u3088\u3046<\/h3>\n\n\n\n<p>\u3055\u3066\u3001\u639b\u3051\u5408\u308f\u305b\u5b9f\u9a13\u306b\u306f\u30aa\u30b9\u3068\u96cc\u96c4\u540c\u4f53\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u5b9f\u9a13\u5ba4\u3067\u98fc\u80b2\u3055\u308c\u3066\u3044\u308b\u7dda\u866b\u306e\u591a\u304f\u306f\u96cc\u96c4\u540c\u4f53\u3067\u3001\u96cc\u96c4\u540c\u4f53\u304b\u3089\u3001\u30aa\u30b9\u306f\u975e\u5e38\u306b\u7a00\u306b\u3057\u304b\u751f\u307e\u308c\u3066\u304d\u307e\u305b\u3093\uff081000\u5339\u306b2\u30013\u5339\u7a0b\u5ea6\uff09\u3002\u305d\u3053\u3067\u3001\u5b9f\u9a13\u5ba4\u3067\u306f\u3001<br>\u30fb\u89aa\u866b\u4e16\u4ee3\u309230\u5ea6\u306e\u9ad8\u6e29\u306b5-6\u6642\u9593\u66b4\u9732\u3059\u308b<br>\u30fb\u89aa\u866b\u4e16\u4ee3\u30927% v\/v \u306e\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u542b\u3080\u30d0\u30c3\u30d5\u30a1\uff08M9\u30d0\u30c3\u30d5\u30a1\uff09\u306b30\u5206\u9593\u66b4\u9732\u3059\u308b\u3002<br>\u3053\u3068\u306b\u3088\u308a\u3001\u5b50\u4e16\u4ee3\u3067\u751f\u307e\u308c\u308b\u30aa\u30b9\u306e\u5272\u5408\u3092\u4e0a\u3052\u3066\u3044\u307e\u3059\u3002\u82e5\u3044\u6210\u866b\u3084L4\u3092\u7528\u3044\u308b\u3068\u3001\u3088\u308a\u30aa\u30b9\u306e\u6bd4\u7387\u304c\u4e0a\u304c\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">3-2. \u639b\u3051\u5408\u308f\u305b\u5b9f\u9a13\u3092\u3057\u3066\u307f\u3088\u3046<\/h3>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p>\u96cc\u96c4\u540c\u4f53\u3068\u30aa\u30b9\u3092\u540c\u3058\u30b7\u30e3\u30fc\u30ec\u3067\u57f9\u990a\u3059\u308b\u3068\u3001\u30aa\u30b9\u3068\u96cc\u96c4\u540c\u4f53\u304c\u4ea4\u5c3e\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u639b\u3051\u5408\u308f\u305b\u304c\u8d77\u3053\u308a\u307e\u3059\u3002\u639b\u3051\u5408\u308f\u305b\u304c\u5931\u6557\u3059\u308b\u3068\u96cc\u96c4\u540c\u4f53\u306e\u5b50\u3057\u304b\u751f\u307e\u308c\u307e\u305b\u3093\u304c\u3001\u639b\u3051\u5408\u308f\u305b\u304c\u3046\u307e\u304f\u884c\u304f\u3068\u5b50\u4f9b\u306e\u7d04\u534a\u6570\u304c\u30aa\u30b9\u306b\u306a\u308b\u306e\u3067\u3001\u639b\u3051\u5408\u308f\u305b\u304c\u6210\u529f\u3057\u305f\u304b\u5931\u6557\u3057\u305f\u304b\u306f\u3059\u3050\u306b\u308f\u304b\u308a\u307e\u3059\u3002\u91ce\u751f\u578b\u306a\u3089\u3070\u3001\u96cc\u96c4\u540c\u4f531\u5339\u306b\u5bfe\u3057\u3001\u5143\u6c17\u306a\u30aa\u30b94\u5339\u307b\u3069\u7f6e\u3044\u3066\u304a\u3051\u3070\u30014\u65e5\u5f8c\u306b\u306f\u30aa\u30b9\u304c\u305f\u304f\u3055\u3093\u3044\u308b\u30d7\u30ec\u30fc\u30c8\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3067\u3057\u3087\u3046\u3002\u3053\u306e\u6761\u4ef6\u3067\u4ea4\u914d\u306b\u5931\u6557\u3057\u305f\u5834\u5408\u306b\u306f\u3001\u30d7\u30ec\u30fc\u30c8\u306b\u5165\u308c\u308b\u30aa\u30b9\u306e\u6570\u3092\u5897\u3084\u3059\u3001\u30d7\u30ec\u30fc\u30c8\u306b\u5857\u308b\u5927\u8178\u83cc\u306e\u9762\u7a4d\u3092\u72ed\u3081\u3066\u30aa\u30b9\u3068\u96cc\u96c4\u540c\u4f53\u304c\u51fa\u4f1a\u3044\u3084\u3059\u304f\u3059\u308b\u3001\u306a\u3069\u306e\u5de5\u592b\u304c\u6709\u52b9\u306a\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"640\" height=\"360\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/experiment1_01.jpg\" alt=\"\" class=\"wp-image-181\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/experiment1_01.jpg 640w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/experiment1_01-300x169.jpg 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><figcaption class=\"wp-element-caption\">\u53c2\u8003\u52d5\u753b\uff1a\u7dda\u866b\u306e\u4ea4\u5c3e\u884c\u52d5\u3000\u592a\u3044\u306e\u304c\u96cc\u96c4\u540c\u4f53\u3001\u305d\u306e\u5468\u308a\u3092\u56de\u3063\u3066\u3044\u308b\u7d30\u3044\u306e\u304c\u30aa\u30b9\u3067\u3059\u3002<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"video\">\r\n<iframe loading=\"lazy\" width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/guEquq7ZExE?si=hemX-147GMCE6heS\" title=\"YouTube video player\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\r\n<\/div>\r\n<style>\r\n.video {\r\n    position: relative;\r\n    width: 100%;\r\n    padding-top: 56.25%; \/* 16:9\u306e\u30a2\u30b9\u30da\u30af\u30c8\u6bd4 *\/\r\n    height: 0;\r\n}\r\n.video iframe {\r\n    position: absolute;\r\n    top: 0;\r\n    left: 0;\r\n    width: 100%;\r\n    height: 100%;\r\n}\r\n<\/style>\n\n\n\n<p class=\"has-text-align-right fs_xs\">\u64ae\u5f71\uff1a\u672b\u5ee3\u52c7\u53f8<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">3-3. \u91ce\u751f\u578b\u3068\u5909\u7570\u4f53\u3092\u639b\u3051\u5408\u308f\u305b\u3066\u307f\u3088\u3046<\/h3>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p><em>dpy-1(tm11382)<\/em>\u5909\u7570\u4f53\u306f\u3001\u91ce\u751f\u578b\u3068\u6bd4\u3079\u308b\u3068\u4f53\u9577\u304c\u77ed\u304f\u3001\u305a\u3093\u3050\u308a\u3080\u3063\u304f\u308a\u3068\u3057\u305f\u5f62\u3092\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u5909\u7570\u306f\u6f5c\u6027\u3067\u3042\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<em>dpy-1(tm11382)<\/em>\u306e\u96cc\u96c4\u540c\u4f53\u3068\u91ce\u751f\u578bN2\u306e\u30aa\u30b9\u3092\u639b\u3051\u5408\u308f\u305b\u308b\u3068\u3069\u3046\u306a\u308b\u3067\u3057\u3087\u3046\u304b\u3002\u5b50\u4e16\u4ee3\u3067\u306f\u3001<em>dpy-1(tm11382)<\/em>\u3092\u30d8\u30c6\u30ed\u3067\u6301\u3064\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u304b\u3089\u3001\u305a\u3093\u3050\u308a\u3080\u3063\u304f\u308a\u306e\u8868\u73fe\u578b\u306f\u6d88\u3048\u3001\u91ce\u751f\u578b\u3068\u540c\u3058\u3088\u3046\u306a\u5f62\u306e\u7dda\u866b\u304c\u51fa\u3066\u304d\u307e\u3059\u3002\u305d\u306e\u5b50\u4e16\u4ee3\u3092\u3055\u3089\u306b\u5358\u96e2\u3057\u3066\u3001\u305d\u306e\u5b50\u4f9b\u3092\u898b\u3066\u307f\u308b\u3068\u30011\/4\u306f\u305a\u3093\u3050\u308a\u3080\u3063\u304f\u308a\u578b\u306b\u30013\/4\u306f\u91ce\u751f\u578b\u3068\u540c\u3058\u3088\u3046\u306a\u5f62\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"300\" height=\"279\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-picture.jpg\" alt=\"\" class=\"wp-image-839\"\/><\/figure>\n<\/div>\n<\/div>\n<\/div><\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<section class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">4.  \u884c\u52d5\u5b9f\u9a13\uff1a\u7dda\u866b\u306e\u597d\u7269\u3092\u63a2\u3057\u3066\u307f\u3088\u3046<\/h2>\n\n\n\n<p>\u7dda\u866b\u306f\u3001\u5302\u3044\u3084\u6e29\u5ea6\u3001\u5869\u306e\u6fc3\u5ea6\u3084\u9178\u7d20\u6fc3\u5ea6\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u74b0\u5883\u60c5\u5831\u3092\u611f\u77e5\u3057\u3001\u6b63\u3084\u8ca0\u306e\u8d70\u6027\u3092\u793a\u3059\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u3001\u7dda\u866b\u304c\u597d\u304d\u306a\u5302\u3044\u3092\u767a\u3059\u308b\u7d0d\u8c46\u3092\u4f8b\u306b\u3068\u3063\u3066\u3001\u3069\u3093\u306a\u5302\u3044\u304c\u597d\u304d\u306a\u306e\u304b\u3001\u307e\u305f\u306f\u5acc\u3044\u306a\u306e\u304b\u3092\u8abf\u3079\u308b\u3001\u7c21\u5358\u306a\u5b9f\u9a13\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">4-1. \u7528\u610f\u3059\u308b\u3082\u306e<\/h3>\n\n\n\n<p>\u6210\u866b\u306e\u7dda\u866b<br>\u30a2\u30c3\u30bb\u30a4\u30d7\u30ec\u30fc\u30c8\uff08\u7d44\u6210\u306f\u5f8c\u8ff0\uff09<br>\u30bc\u30e9\u30c1\u30f3\u5165\u308aM9\u30d0\u30c3\u30d5\u30a1\uff08\u7d44\u6210\u306f\u5f8c\u8ff0\uff09<br>1.5 ml\u30c1\u30e5\u30fc\u30d6<br>\u7d0d\u8c46<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">4-2. \u5b9f\u9a13\u624b\u9806<\/h3>\n\n\n\n<div class=\"wp-block-columns box_col_02 is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-top is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:60%\">\n<p>(1) \u5927\u8178\u83cc\u3092\u5857\u3063\u305f6 cm\u30d7\u30ec\u30fc\u30c8\u306b\u91ce\u751f\u578b\u7dda\u866b\u30922\u5339\u7a0b\u5ea6\u7f6e\u304d\u300120\u5ea6\u30673-4\u65e5\u9593\u3001\u5b50\u4f9b\u304c\u6210\u866b\u306b\u306a\u308b\u307e\u3067\u57f9\u990a\u3057\u307e\u3059\u3002<br>(2) \u7dda\u866b\u304c\u6210\u866b\u307e\u3067\u80b2\u3063\u305f\u3089\u3001\u5b9f\u9a13\u958b\u59cb\u3067\u3059\u3002\u307e\u305a\u3001\u30a2\u30c3\u30bb\u30a4\u30d7\u30ec\u30fc\u30c8\u306e\u6e96\u5099\u3092\u3057\u307e\u3059\u3002\u30a2\u30c3\u30bb\u30a4\u30d7\u30ec\u30fc\u30c8\u306e\u4e2d\u592e\u3068\u4e21\u7aef\u306b\u30de\u30b8\u30c3\u30af\u3067\u5370\u3092\u3064\u3051\u3001\u4e00\u65b9\u306e\u7aef\u306b\u7d0d\u8c46\u3092\u7f6e\u304d\u307e\u3059\u3002<br>(3) \u6b21\u306b\u3001\u7dda\u866b\u3092\u56de\u53ce\u3057\u307e\u3059\u3002\u30bc\u30e9\u30c1\u30f3\u5165\u308aM9\u30d0\u30c3\u30d5\u30a11ml\u7a0b\u5ea6\u3092\u7528\u3044\u3066\u3001\u7dda\u866b\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u7dda\u866b\u30921.5 ml\u30c1\u30e5\u30fc\u30d6\u306b\u56de\u53ce\u3057\u307e\u3059\u3002\u3053\u306e\u969b\u7528\u3044\u308b\u306e\u306f\u30d1\u30b9\u30c4\u30fc\u30eb\u30d4\u30da\u30c3\u30c8\u3001\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u306a\u3069\u306a\u3093\u3067\u3082\u69cb\u3044\u307e\u305b\u3093\u3002<br>(4) 1\u5206\u307b\u3069\u5f85\u3063\u3066\u7dda\u866b\u304c1.5 ml\u30c1\u30e5\u30fc\u30d6\u306e\u5e95\u306b\u6c88\u3093\u3060\u3089\u3001\u4e0a\u6e05\u3060\u3051\u3092\u56de\u53ce\u3057\u3066\u6368\u3066\u3001\u65b0\u3057\u3044M9\u30d0\u30c3\u30d5\u30a1\u30921.5 ml\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u3066\u866b\u3092\u6d17\u6d44\u3057\u307e\u3059\u3002\u3053\u308c\u30922\u56de\u7e70\u308a\u8fd4\u3057\u3001\u5927\u8178\u83cc\u3092\u306a\u308b\u3079\u304f\u53d6\u308a\u9664\u304d\u307e\u3059\u3002\u203b\u3053\u3053\u3067\u3001\u4e0a\u6e05\u3092\u6368\u3066\u305f\u5ec3\u6db2\u306b\u306f\u7dda\u866b\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\u306a\u3069\u3067\u304d\u3061\u3093\u3068\u7dda\u866b\u3092\u6bba\u3057\u3066\u304b\u3089\u6368\u3066\u308b\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br>(5) \u5e95\u306b\u6c88\u3093\u3060\u7dda\u866b\u3092\u306a\u308b\u3079\u304f\u5438\u3044\u53d6\u308b\u30d0\u30c3\u30d5\u30a1\u304c\u5c11\u306a\u304f\u306a\u308b\u3088\u3046\u306b\u30d4\u30da\u30c3\u30c8\u3067\u56de\u53ce\u3057\u3001\u30a2\u30c3\u30bb\u30a4\u30d7\u30ec\u30fc\u30c8\u306e\u4e2d\u592e\u306b\u7f6e\u304d\u3001\u30da\u30fc\u30d1\u30fc\u30bf\u30aa\u30eb\u306a\u3069\u3067\u6c34\u6c17\u3092\u62ed\u304d\u53d6\u308a\u307e\u3059\u3002<br>(6) \u84cb\u3092\u3057\u3066\u3001\u5b9f\u9a13\u30b9\u30bf\u30fc\u30c8\uff01\u7dda\u866b\u304c\u7d0d\u8c46\u306b\u5bc4\u3063\u3066\u884c\u304f\u69d8\u5b50\u3092\u89b3\u5bdf\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column sp_box_ord_01 is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:40%\"><div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"450\" height=\"406\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-2picture.jpg\" alt=\"\" class=\"wp-image-840\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-2picture.jpg 450w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-2picture-300x271.jpg 300w\" sizes=\"(max-width: 450px) 100vw, 450px\" \/><\/figure>\n<\/div><\/div>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">4-3. \u767a\u5c55\u7de8<\/h3>\n\n\n\n<p>\u7d0d\u8c46\u306e\u4ee3\u308f\u308a\u306b\u30b3\u30fc\u30d2\u30fc\u3084\u30cf\u30f3\u30c9\u30bd\u30fc\u30d7\u306a\u3069\u3001\u8abf\u3079\u305f\u3044\u3082\u306e\u3092\u8272\u3005\u3068\u7f6e\u3044\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u7dda\u866b\u306f\u3069\u3093\u306a\u3082\u306e\u304c\u597d\u304d\u3067\u3057\u3087\u3046\u304b\u3002\u307e\u305f\u3001\u5909\u7570\u4f53\u3092\u4f7f\u3063\u3066\u307f\u305f\u3089\u3001\u3069\u3093\u306a\u884c\u52d5\u3092\u793a\u3059\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full is-resized\"><img decoding=\"async\" width=\"800\" height=\"616\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-3pic.jpg\" alt=\"\" class=\"wp-image-856\" style=\"width:800px;height:auto\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-3pic.jpg 800w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-3pic-300x231.jpg 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-3pic-768x591.jpg 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/4-1-3pic-670x516.jpg 670w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>\u76e3\u4fee\u8005<\/strong><br>\u51fa\u5d8b\u3000\u514b\u53f2&nbsp;<br>\u6771\u4eac\u5973\u5b50\u533b\u79d1\u5927\u5b66<\/p>\n\n\n\n<p><strong>\u7814\u7a76\u5ba4HP<\/strong><br><a href=\"https:\/\/researchmap.jp\/dejimakatsufumi\">https:\/\/researchmap.jp\/dejimakatsufumi<\/a><\/p>\n\n\n\n<p>\u9152\u4e95\u3000\u5948\u7dd2\u5b50<br>\u30a2\u30e1\u30ea\u30ab\u5408\u8846\u56fd\u30d4\u30c3\u30c4\u30d0\u30fc\u30b0\u751f\u307e\u308c\u3002\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662\u535a\u58eb\u8ab2\u7a0b\u4fee\u4e86\u3002\u3055\u307e\u3056\u307e\u306a\u5207\u308a\u53e3\u304b\u3089\u751f\u547d\u73fe\u8c61\u3092\u7814\u7a76\u3067\u304d\u308b\u7dda\u866b\u907a\u4f1d\u5b66\u306b\u9b45\u305b\u3089\u308c\u3066\u7814\u7a76\u3092\u7d9a\u3051\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>\u7814\u7a76\u5ba4HP<\/strong><br><a href=\"https:\/\/researchmap.jp\/n-sakai\">https:\/\/researchmap.jp\/n-sakai<\/a><\/p>\n\n\n\n<p class=\"has-text-align-right\"><a href=\"#toc_container\">\u76ee\u6b21\u30da\u30fc\u30b8\u3078\u2191<\/a><\/p>\n<\/div><\/section>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>C. elegans\u3092\u4f7f\u3046\u3053\u3068\u3067\u3001\u907a\u4f1d\u5b50\u306e\u5909\u7570\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u7570\u5e38\u30fb\u8868\u73fe\u578b\u304c\u751f\u3058\u308b\u3068\u3044\u3046\u3053\u3068\u3092\u76f4\u63a5\u89b3\u5bdf [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":864,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"[{\"id\":\"79584c0e-1740-4068-a550-a5ca21b79c06\",\"content\":\"\"}]"},"class_list":["post-892","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/892","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/comments?post=892"}],"version-history":[{"count":2,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/892\/revisions"}],"predecessor-version":[{"id":1046,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/892\/revisions\/1046"}],"up":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/864"}],"wp:attachment":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/media?parent=892"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}