{"id":889,"date":"2025-03-21T11:55:59","date_gmt":"2025-03-21T02:55:59","guid":{"rendered":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/?page_id=889"},"modified":"2025-04-01T10:52:22","modified_gmt":"2025-04-01T01:52:22","slug":"info2-handling","status":"publish","type":"page","link":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/info-2\/info2-handling\/","title":{"rendered":"\u7dda\u866b\u306e\u57fa\u672c\u7684\u306a\u53d6\u308a\u6271\u3044"},"content":{"rendered":"\n<div id=\"toc_container\" class=\"wp-block-group pada_30\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">\u76ee\u6b21<\/h2>\n\n\n\n<ul class=\"wp-block-list list_none has-medium-font-size\">\n<li><strong><a href=\"#info2-midashi01\" data-type=\"internal\" data-id=\"#section-1\">\u5fc5\u8981\u306a\u8a2d\u5099<\/a><\/strong><\/li>\n\n\n\n<li><strong><a href=\"#info2-midashi02\" data-type=\"internal\" data-id=\"#section-1\">\u901a\u5e38\u306e\u98fc\u80b2\u65b9\u6cd5<\/a><\/strong><\/li>\n\n\n\n<li><strong><a href=\"#info2-midashi03\">\u30d4\u30c3\u30ab\u30fc\u306e\u4f5c\u6210\u65b9\u6cd5<\/a><\/strong><\/li>\n\n\n\n<li><strong><a href=\"#info2-midashi04\"><strong>\u7dda\u866b\u306e\u904b\u642c\u30fb\u5ec3\u68c4<\/strong><\/a><\/strong><\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<div id=\"info2-midashi01\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">1.<strong>&nbsp; <strong>\u5fc5\u8981\u306a\u8a2d\u5099<\/strong><\/strong><\/h2>\n\n\n\n<p>\u7dda\u866b\uff08<em>Caenorhabditis elegans<\/em> \u4ee5\u4e0b\u7dda\u866b\u3068\u547c\u3076\uff09\u98fc\u80b2\u306b\u5fc5\u8981\u306a\u8a2d\u5099\u306b\u306f\u3001\u7dda\u866b\u98fc\u80b2\u7528\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u3001\u57f9\u5730\u7528\u8a66\u85ac\u3092\u6eb6\u89e3\u3059\u308b\u305f\u3081\u306e\u52a0\u71b1\u6a5f\u5668\u3001\u305d\u3057\u3066\u89b3\u5bdf\u7528\u30c4\u30fc\u30eb\u304c\u3042\u3052\u3089\u308c\u307e\u3059\u3002\u57f9\u5730\u4f5c\u6210\u7528\u306e\u52a0\u71b1\u6eb6\u89e3\u306f\u96fb\u5b50\u30ec\u30f3\u30b8\u3067\u3082\u4ee3\u7528\u3067\u304d\u307e\u3059\u3002\u305f\u3060\u3057\u3001\u7dda\u866b\u30d7\u30ec\u30fc\u30c8\u3092\u5ec3\u68c4\u3059\u308b\u969b\u306b\u306f\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\u304c\u5fc5\u8981\u3068\u306a\u308b\u305f\u3081\u3001\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\u3092\u6e96\u5099\u3059\u308b\u306e\u304c\u671b\u307e\u3057\u3044\u3067\u3057\u3087\u3046\u3002\u98fc\u80b2\u7528\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306f\u3001\u53b3\u5bc6\u306a\u5b9f\u9a13\u3067\u306a\u3051\u308c\u3070\u3001\u30ef\u30a4\u30f3\u30af\u30fc\u30e9\u30fc\u306a\u3069\u3067\u3082\u5341\u5206\u4ee3\u7528\u3067\u304d\u307e\u3059\u3002\u89b3\u5bdf\u7528\u30c4\u30fc\u30eb\u306f\u3001\u643a\u5e2f\u96fb\u8a71\u30ab\u30e1\u30e9\u3067\u306f\u6210\u866b\u306e\u78ba\u8a8d\u304c\u3067\u304d\u308b\u7a0b\u5ea6\u3067\u3059\u304c\u3001\u5b9f\u4f53\u9855\u5fae\u93e1\u3092\u4f7f\u7528\u3059\u308c\u3070\u4f53\u306e\u5185\u90e8\u69cb\u9020\u307e\u3067\u89b3\u5bdf\u3067\u304d\u308b\u305f\u3081\u3001\u5f37\u304f\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u5b9f\u4f53\u9855\u5fae\u93e1\u306e\u500d\u7387\u306f10-50\u500d\u7a0b\u5ea6\u3042\u308c\u3070\u826f\u3044\u3067\u3057\u3087\u3046\u3002\u900f\u660e\u306a\u7dda\u866b\u306e\u4f53\u304c\u3088\u304f\u898b\u3048\u308b\u3088\u3046\u306b\u3001\u900f\u904e\u578b\u3001\u504f\u659c\u7167\u660e\u306e\u5b9f\u4f53\u9855\u5fae\u93e1\u304c\u5b9f\u9a13\u5ba4\u3067\u306f\u3088\u304f\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-image mb_80\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"1218\" height=\"748\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_01.png\" alt=\"\" class=\"wp-image-990\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_01.png 1218w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_01-300x184.png 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_01-1024x629.png 1024w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_01-768x472.png 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_01-670x411.png 670w\" sizes=\"(max-width: 1218px) 100vw, 1218px\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>\u7dda\u866b\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u4f5c\u6210\u65b9\u6cd5<\/strong><\/h3>\n\n\n\n<p>\u7dda\u866b\u306e\u98fc\u80b2\u306b\u306fNGM(Nematode Growth Medium)\u3068\u3088\u3070\u308c\u308b\u5bd2\u5929\u57f9\u5730\u3092\u7528\u3044\u307e\u3059\u3002NGM\u4e0a\u306b\u990c\u3068\u306a\u308b\u5927\u8178\u83ccOP50\u682a\u3092\u64ad\u7a2e\u3057\u3066\u4e7e\u304b\u3057\u305f\u5f8c\u3001\u305d\u306e\u4e0a\u306b\u7dda\u866b\u3092\u4e57\u305b\u3066\u98fc\u80b2\u3059\u308b\u306e\u304c\u4e00\u822c\u7684\u306a\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image mb_80\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"875\" height=\"497\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_02.png\" alt=\"\" class=\"wp-image-992\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_02.png 875w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_02-300x170.png 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_02-768x436.png 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/equipment_02-670x381.png 670w\" sizes=\"(max-width: 875px) 100vw, 875px\" \/><\/figure>\n<\/div>\n\n\n<div class=\"wp-block-group is-layout-grid wp-container-core-group-is-layout-baef362d wp-block-group-is-layout-grid\">\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\"><strong>NGM\u306e\u7d44\u6210(1 L)<\/strong><\/h4>\n\n\n\n<figure class=\"wp-block-flexible-table-block-table alignleft is-content-justification-center\"><table class=\"has-fixed-layout is-stacked-on-mobile\" style=\"max-width:100%;width:100%\"><tbody><tr><td style=\"width:75%;border-style:dashed\">NaCl<\/td><td style=\"text-align:right;border-style:dashed\">3 g<\/td><\/tr><tr><td style=\"width:75%;border-style:dashed\">Bacto Peptone<\/td><td style=\"text-align:right;border-style:dashed\">2.5 g<\/td><\/tr><tr><td style=\"width:75%;border-style:dashed\">Agar<\/td><td style=\"text-align:right;border-style:dashed\">17 g<\/td><\/tr><tr><td style=\"border-style:dashed dashed solid\">\u84b8\u7559\u6c34<\/td><td style=\"text-align:right;border-style:dashed dashed solid\">972 ml<\/td><\/tr><tr><td style=\"border-style:dashed\">\u3000<\/td><td style=\"text-align:right;border-style:dashed\">\u3000<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"has-text-align-left\">\u2193\u9ad8\u71b1\u6eb6\u89e3<br>\u219360\u2103\u4ee5\u4e0b\u306b\u306a\u3063\u305f\u3089\u3001\u4e0b\u8a18\u6dfb\u52a0\u3059\u308b<\/p>\n\n\n\n<figure class=\"wp-block-flexible-table-block-table alignleft is-content-justification-center\"><table class=\"has-fixed-layout is-stacked-on-mobile\" style=\"max-width:100%;width:100%\"><tbody><tr><td style=\"width:75%;border-style:dashed\">1M \u30ea\u30f3\u9178\u30ab\u30ea\u30a6\u30e0\u7de9\u885d\u6db2\uff08pH 6.0\uff09<\/td><td style=\"text-align:right;border-style:dashed\">25 ml<\/td><\/tr><tr><td style=\"width:75%;border-style:dashed\">1M MgSO<sub>4<\/sub><\/td><td style=\"text-align:right;border-style:dashed\">1 ml<\/td><\/tr><tr><td style=\"width:75%;border-style:dashed\">1M CaCl<sub>2<\/sub><\/td><td style=\"text-align:right;border-style:dashed\">1 ml<\/td><\/tr><tr><td style=\"border-style:dashed dashed solid\">5 mg\/ml cholesterol&nbsp;&nbsp;&nbsp;&nbsp;<\/td><td style=\"text-align:right;border-style:dashed dashed solid\">1 ml<\/td><\/tr><tr><td style=\"border-style:dashed\">\u3000<\/td><td style=\"text-align:right;border-style:dashed\">\u3000<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"has-text-align-left\">\u2193 dish\u306b\u5206\u6ce8\u3059\u308b\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\"><strong>1M \u30ea\u30f3\u9178\u30ab\u30ea\u30a6\u30e0\u7de9\u885d\u6db2\u306e\u7d44\u6210<\/strong><\/h4>\n\n\n\n<figure class=\"wp-block-flexible-table-block-table alignleft is-content-justification-center\"><table class=\"has-fixed-layout is-stacked-on-mobile\" style=\"max-width:100%;width:100%\"><tbody><tr><td style=\"width:75%;border-style:dashed\">KH<sub>2<\/sub>PO<sub>4<\/sub>(\u7121\u6c34)<\/td><td style=\"text-align:right;border-style:dashed\">108.3 g<\/td><\/tr><tr><td style=\"width:75%;border-style:dashed\">K<sub>2<\/sub>HPO<sub>4<\/sub>(\u7121\u6c34)<\/td><td style=\"text-align:right;border-style:dashed\">35.6 g<\/td><\/tr><tr><td style=\"border-style:dashed dashed solid\">\u84b8\u7559\u6c34<\/td><td style=\"text-align:right;border-style:dashed dashed solid\">to 1 L<\/td><\/tr><tr><td style=\"border-style:dashed\">\u3000<\/td><td style=\"text-align:right;border-style:dashed\">1 L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"has-text-align-left\">\u2193 \u9ad8\u71b1\u6eb6\u89e3<br>\u2193 \u5ba4\u6e29\u4fdd\u5b58<\/p>\n\n\n\n<p>*\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u306f100\uff05\u30a8\u30bf\u30ce\u30fc\u30eb\u306b\u6eb6\u89e3\u3059\u308b\uff08\u6ec5\u83cc\u4e0d\u8981\uff09\u3002\u6eb6\u3051\u306b\u304f\u3044\u306e\u3067\u3001NGM\u8abf\u6574\u306e\u524d\u65e5\u306b\u7528\u610f\u3057\u3066\u304a\u304f\u3002<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>1L\u30676 cm\u30d7\u30ec\u30fc\u30c8\u304c\u304a\u3088\u305d120\u679a\u7a0b\u5ea6\u4f5c\u6210\u3067\u304d\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u306b\u6ce8\u3050\u5bd2\u5929\u57f9\u5730\u306e\u91cf\u306f\u9069\u91cf\u3067\u3059\u304c\u30013.5 cm\u30849 cm\u306a\u3069\u7570\u306a\u308b\u30b5\u30a4\u30ba\u306e\u30d7\u30ec\u30fc\u30c8\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u5bd2\u5929\u57f9\u5730\u306e\u539a\u307f\u3092\u5747\u4e00\u306b\u3059\u308b\u3053\u3068\u3067\u3001\u9855\u5fae\u93e1\u89b3\u5bdf\u6642\u306b\u30d4\u30f3\u30c8\u3092\u5408\u308f\u305b\u308b\u624b\u9593\u304c\u7701\u3051\u3066\u4fbf\u5229\u3067\u3059\u3002NBRP\u3067\u306f\u30013.5 cm\u30d7\u30ec\u30fc\u30c8\u306b\u306f3.8 ml, 6cm\u30d7\u30ec\u30fc\u30c8\u306b8 ml, 9 cm\u30d7\u30ec\u30fc\u30c8\u306b\u306f24 ml\u3092\u6a19\u6e96\u3068\u3057\u3066\u5bd2\u5929\u57f9\u5730\u3092\u6492\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u5927\u8178\u83cc\u306e\u64ad\u7a2e\u306f\u3001\u5927\u8178\u83cc\u6db2\u3092NGM\u30d7\u30ec\u30fc\u30c8\u306b\u5782\u3089\u3057\u3001\u57f9\u990a\u3059\u308b\u3053\u3068\u3067\u884c\u3044\u307e\u3059\u3002\u30b9\u30d7\u30ec\u30c3\u30bf\u30fc\u3092\u4f7f\u7528\u3057\u3066\u5857\u308a\u5e83\u3052\u308b\u65b9\u6cd5\u3067\u3082\u3001\u30d7\u30ec\u30fc\u30c8\u3092\u50be\u3051\u3066\u5927\u8178\u83cc\u6db2\u3092\u5e83\u3052\u308b\u65b9\u6cd5\u3067\u3082\u826f\u3044\u3067\u3059\u304c\u3001\u5927\u8178\u83cc\u53e2\u304c\u30d7\u30ec\u30fc\u30c8\u306e\u7aef\u306b\u89e6\u308c\u306a\u3044\u3088\u3046\u306b\u3057\u305f\u65b9\u304c\u3044\u3044\u3067\u3057\u3087\u3046\u3002\u7aef\u306b\u5927\u8178\u83cc\u304c\u3042\u308b\u3068\u305d\u3053\u304b\u3089\u7dda\u866b\u304c\u30d7\u30ec\u30fc\u30c8\u3068\u5bd2\u5929\u306e\u9593\u306b\u6f5c\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n\n\n\n<div id=\"info2-midashi02\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">2.<strong>&nbsp; <strong>\u901a\u5e38\u306e\u98fc\u80b2\u65b9\u6cd5<\/strong><\/strong><\/h2>\n\n\n\n<p>\u7dda\u866b\u306f12\u2103\u30fc25\u2103\u304f\u3089\u3044\u306e\u6e29\u5ea6\u5e2f\u3067\u6210\u9577\u3001\u5897\u6b96\u304c\u53ef\u80fd\u3067\u3059\u3002\u4e00\u822c\u7684\u306b\u3001\u7814\u7a76\u5ba4\u3067\u306f20\u2103\u306b\u8a2d\u5b9a\u3057\u305f\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u3067\u7dda\u866b\u3092\u98fc\u80b2\u3057\u3066\u3044\u307e\u3059\u3002\u91ce\u751f\u578b\u306e\u7dda\u866b\u306f15\u2103\u30674\u65e5\u300120\u2103\u30673\u65e5\u300125\u2103\u30672.5\u65e5\u307b\u3069\u3067\u5375\u304b\u3089\u6210\u866b\u306b\u306a\u308a\u3001\u5375\u3092\u7523\u307f\u59cb\u3081\u307e\u3059\u3002\u653e\u3063\u3066\u304a\u304f\u3068\u990c\u3068\u306a\u308b\u5927\u8178\u83cc\u304c\u67af\u6e07\u3057\u3066\u3057\u307e\u3046\u306e\u3067\u3001\u5b9a\u671f\u7684\u306b\u65b0\u3057\u3044\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u306b\u7d99\u4ee3\u3057\u3066\u98fc\u80b2\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u7d99\u4ee3\u306e\u969b\u306b\u306f\u3001\u30d4\u30c3\u30ab\u30fc\u3068\u547c\u3070\u308c\u308b\u6ec5\u83cc\u53ef\u80fd\u306a\u767d\u91d1\u7dda(\u4f5c\u308a\u65b9\u306f\u5f8c\u8ff0)\u3067\u7dda\u866b\u3092\u540a\u308a\u4e0a\u3052\u3001\u65b0\u3057\u3044\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u306b\u79fb\u3057\u307e\u3059\u3002\u990c\u304c\u306a\u304f\u306a\u3063\u305f\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u30b2\u30eb\u7247\u3092\u5207\u308a\u53d6\u3063\u3066\u65b0\u3057\u3044\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u306b\u79fb\u3059\u3053\u3068\u3067\u3082\u7d99\u4ee3\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u7dda\u866b\u304c\u3044\u308b\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u306f\u3001\u4e7e\u71e5\u3092\u9632\u3050\u305f\u3081\u306b\u5bc6\u9589\u5bb9\u5668\u306b\u5165\u308c\u3066\u4fdd\u7ba1\u3059\u308b\u306e\u304c\u671b\u307e\u3057\u3044\u3067\u3059\u3002NBRP\u3067\u306f\u84cb\u3064\u304d\u306e\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u30b1\u30fc\u30b9\u306b\u5165\u308c\u3066\u7dda\u866b\u3092\u98fc\u80b2\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"1260\" height=\"381\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon1.jpg\" alt=\"\" class=\"wp-image-997\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon1.jpg 1260w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon1-300x91.jpg 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon1-1024x310.jpg 1024w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon1-768x232.jpg 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon1-670x203.jpg 670w\" sizes=\"(max-width: 1260px) 100vw, 1260px\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><strong><strong><strong>\u7dda\u866b\u306e\u4fdd\u5b58<\/strong><\/strong><\/strong><\/h3>\n\n\n\n<p>\u9577\u671f\u9593\u4f7f\u7528\u3057\u306a\u3044\u5834\u5408\u306b\u306f\u3001\u30c0\u30a6\u30a2\u30fc\u5e7c\u866b\uff08\u8010\u6027\u5e7c\u866b\uff09\u306e\u72b6\u614b\u3067\u6570\u30f6\u6708\u4fdd\u7ba1\u3059\u308b\u65b9\u6cd5\u3068\u3001\u6db2\u4f53\u7a92\u7d20\u3084-80\u2103\u3067\u534a\u6c38\u4e45\u7684\u306b\u4fdd\u5b58\u3059\u308b\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">\u30c0\u30a6\u30a2\u30fc\u5e7c\u866b\u3067\u4fdd\u5b58\u3059\u308b\u65b9\u6cd5<\/h5>\n\n\n\n<p>\u7dda\u866b\u306f\u98e2\u9913\u72b6\u614b\u3084\u904e\u5bc6\u72b6\u614b\u306a\u3069\u3001\u751f\u5b58\u306b\u4e0d\u5229\u306a\u74b0\u5883\u306b\u304a\u304b\u308c\u308b\u3068\u3001\u30c0\u30a6\u30a2\u30fc\u5e7c\u866b\uff08\u8010\u6027\u5e7c\u866b\uff09\u3068\u547c\u3070\u308c\u308b\u72b6\u614b\u306b\u306a\u308a\u3001\u990c\u304c\u306a\u304f\u3066\u3082\u6570\u30f6\u6708\u751f\u304d\u7d9a\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u96cc\u96c4\u540c\u4f531\u5339\u304c\u3044\u308c\u3070\u6b21\u4e16\u4ee3\u3092\u6b8b\u305b\u308b\u305f\u3081\u3001\u6bd4\u8f03\u7684\u5bb9\u6613\u306b\u4fdd\u5b58\u304c\u53ef\u80fd\u3067\u3059\u3002\u98e2\u9913\u72b6\u614b\u306b\u306a\u308b\u3068\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u9038\u8d70\u3059\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u307e\u308b\u305f\u3081\u3001\u307e\u305f\u3001\u4e7e\u71e5\u3092\u9632\u3050\u305f\u3081\u3001\u30d1\u30e9\u30d5\u30a3\u30eb\u30e0\u304b\u98df\u54c1\u7528\u30e9\u30c3\u30d7\u30d5\u30a3\u30eb\u30e0\u306b\u5305\u3093\u3067\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u5185\u3067\u98fc\u80b2\u3059\u308b\u306e\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u653e\u7f6e\u3057\u3066\u3044\u3066\u3082\u3001\u6570\u30f6\u6708\u306b\u4e00\u5ea6\u3001\u30c0\u30a6\u30a2\u30fc\u7dda\u866b\u304c\u3044\u308b\u5bd2\u5929\u7247\u3092\u5207\u308a\u53d6\u3063\u3066\u65b0\u3057\u3044\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u306b\u79fb\u3059\u3053\u3068\u3067\u3001\u98fc\u80b2\u3092\u7d9a\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">\u6db2\u4f53\u7a92\u7d20\u3084-80\u2103\u3067\u534a\u6c38\u4e45\u7684\u306b\u4fdd\u5b58\u3059\u308b\u65b9\u6cd5<\/h5>\n\n\n\n<p>\u3088\u308a\u5927\u304d\u306a\u8a2d\u5099\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u30c7\u30a3\u30fc\u30d7\u30d5\u30ea\u30fc\u30b6\u30fc\u3084\u6db2\u4f53\u7a92\u7d20\u306a\u3069\u3092\u7528\u3044\u308c\u3070\u3001\u534a\u6c38\u4e45\u7684\u306b\u7dda\u866b\u3092\u4fdd\u5b58\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u51cd\u7d50\u4fdd\u8b77\u5264\u3068\u3057\u3066\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u3084\u30c8\u30ec\u30cf\u30ed\u30fc\u30b9\u304c\u5165\u3063\u305f\u51cd\u7d50\u4fdd\u5b58\u6db2\u3092\u7528\u3044\u3066\u3001\u767a\u6ce1\u30b9\u30c1\u30ed\u30fc\u30eb\u5bb9\u5668\u306a\u3069\u3092\u7528\u3044\u3066\u7de9\u6162\u51b7\u5374\u3059\u308b\u3053\u3068\u3067\u3001\u8d85\u4f4e\u6e29\u3067\u534a\u6c38\u4e45\u7684\u306b\u4fdd\u5b58\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"1344\" height=\"359\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon2.png\" alt=\"\" class=\"wp-image-998\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon2.png 1344w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon2-300x80.png 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon2-1024x274.png 1024w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon2-768x205.png 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon2-670x179.png 670w\" sizes=\"(max-width: 1344px) 100vw, 1344px\" \/><\/figure>\n<\/div>\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"848\" height=\"374\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon3.png\" alt=\"\" class=\"wp-image-999\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon3.png 848w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon3-300x132.png 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon3-768x339.png 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2025\/03\/1-1-1-hozon3-670x295.png 670w\" sizes=\"(max-width: 848px) 100vw, 848px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-right\"><a href=\"#toc_container\">\u76ee\u6b21\u30da\u30fc\u30b8\u3078\u2191<\/a><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n\n\n\n<div id=\"info2-midashi03\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">3. \u30d4\u30c3\u30ab\u30fc\u306e\u4f5c\u6210\u65b9\u6cd5<\/h2>\n\n\n\n<p>\u7dda\u866b\u3092\u65b0\u3057\u3044\u98fc\u80b2\u30d7\u30ec\u30fc\u30c8\u306b\u79fb\u3059\u969b\u306b\u306f\u3001\u767d\u91d1\u7dda\u3067\u3067\u304d\u305f\u7dda\u866b\u30d4\u30c3\u30ab\u30fc\u3092\u7528\u3044\u307e\u3059\u3002\u7dda\u866b\u30d4\u30c3\u30ab\u30fc\u306e\u4f5c\u308a\u65b9\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u3067\u3059\u3002\u30d1\u30b9\u30c4\u30fc\u30eb\u30d4\u30da\u30c3\u30c8\u3068\u767d\u91d1\u7dda\u3092\u7528\u3044\u3066\u7c21\u4fbf\u306b\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u30010.25 mm\u306e\u767d\u91d1\u7dda\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full is-resized\"><img decoding=\"async\" width=\"1054\" height=\"534\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_04.jpg\" alt=\"\" class=\"wp-image-153\" style=\"width:850px\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_04.jpg 1054w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_04-300x152.jpg 300w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_04-1024x519.jpg 1024w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_04-768x389.jpg 768w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_04-670x339.jpg 670w\" sizes=\"(max-width: 1054px) 100vw, 1054px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-right\"><a href=\"#toc_container\">\u76ee\u6b21\u30da\u30fc\u30b8\u3078\u2191<\/a><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n\n\n\n<div id=\"info2-midashi04\" class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">4. <strong>\u7dda\u866b\u306e\u904b\u642c\u30fb\u5ec3\u68c4<\/strong><\/h2>\n\n\n\n<p>\u5b9f\u9a13\u3067\u751f\u7269\u3092\u6271\u3046\u3068\u304d\u306f\u3001\u5b9f\u9a13\u5ba4\u5185\u3067\u98fc\u80b2\u30fb\u4fdd\u7ba1\u3057\u3066\u3044\u308b\u751f\u7269\u304c\u81ea\u7136\u754c\u306b\u51fa\u308b\u3053\u3068\u304c\u306a\u3044\u3088\u3046\u306b\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u7dda\u866b\u3092\u5b9f\u9a13\u5ba4\u306e\u5916\u306b\u6301\u3061\u51fa\u3057\u305f\u308a\u5ec3\u68c4\u3057\u305f\u308a\u3059\u308b\u5834\u5408\u306f\u3001\u305d\u306e\u3053\u3068\u306b\u6ce8\u610f\u3057\u3066\u9069\u5207\u306b\u53d6\u308a\u6271\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">\u904b\u642c<\/h5>\n\n\n\n<p>\u98fc\u80b2\u3057\u3066\u3044\u308b\u30b7\u30e3\u30fc\u30ec\u306e\u5468\u56f2\u3092\u30d1\u30e9\u30d5\u30a3\u30eb\u30e0\u3084\u30c6\u30fc\u30d7\u3067\u3075\u3055\u304e\u3001\u3055\u3089\u306b\u4e08\u592b\u306a\u5bb9\u5668\u306b\u3044\u308c\u3066\u904b\u642c\u3057\u307e\u3059\uff08\u5199\u771f\uff09\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">\u5ec3\u68c4<\/h5>\n\n\n\n<p>\u98fc\u80b2\u3057\u3066\u3044\u308b\u30b7\u30e3\u30fc\u30ec\u3054\u3068\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\uff08\u9ad8\u5727\u84b8\u6c17\u6ec5\u83cc\u5668\uff09\u306b\u304b\u3051\u3001120\u2103\u306720\u5206\u51e6\u7406\u3057\u3066\u6b7b\u6ec5\u3055\u305b\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group mrl_auto w_80\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<div class=\"wp-block-columns is-not-stacked-on-mobile mb_0 is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"472\" height=\"329\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_06.jpg\" alt=\"\" class=\"wp-image-161\" style=\"width:auto;height:235px\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_06.jpg 472w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_06-300x209.jpg 300w\" sizes=\"(max-width: 472px) 100vw, 472px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"336\" height=\"404\" src=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_05.jpg\" alt=\"\" class=\"wp-image-162\" style=\"width:auto;height:235px\" srcset=\"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_05.jpg 336w, https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-content\/uploads\/2024\/12\/equipment_05-250x300.jpg 250w\" sizes=\"(max-width: 336px) 100vw, 336px\" \/><\/figure>\n<\/div>\n<\/div>\n<\/div><\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h5 class=\"wp-block-heading\"><strong>\u53c2\u8003\u8cc7\u6599<\/strong><\/h5>\n\n\n\n<p>Stiernagle, T. Maintenance of C. elegans (February 11, 2006), WormBook, ed. The C. elegans Research Community, WormBook, doi\/10.1895\/wormbook.1.101.1, <a href=\"http:\/\/www.wormbook.org\/\">http:\/\/www .wormbook.org<\/a><\/p>\n\n\n\n<h5 class=\"wp-block-heading\"><strong>\u76e3\u4fee\u8005<\/strong><\/h5>\n\n\n\n<p>\u5ee3\u7530\u3000\u6075\u5b50\uff08\u6771\u4eac\u5973\u5b50\u533b\u79d1\u5927\u5b66\u3000\u533b\u5b66\u90e8\uff09<br>\u5409\u540d\u3000\u4f50\u548c\u5b50\uff08\u6771\u4eac\u5973\u5b50\u533b\u79d1\u5927\u5b66\u3000\u533b\u5b66\u90e8\uff09<br>\u5409\u7530\u3000\u6176\u592a\uff08\u6771\u4eac\u5973\u5b50\u533b\u79d1\u5927\u5b66\u3000\u533b\u5b66\u90e8\uff09<\/p>\n\n\n\n<p class=\"has-text-align-right\"><a href=\"#toc_container\">\u76ee\u6b21\u30da\u30fc\u30b8\u3078\u2191<\/a><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u76ee\u6b21 1.&nbsp; \u5fc5\u8981\u306a\u8a2d\u5099 \u7dda\u866b\uff08Caenorhabditis elegans \u4ee5\u4e0b\u7dda\u866b\u3068\u547c [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":864,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-889","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/889","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/comments?post=889"}],"version-history":[{"count":1,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/889\/revisions"}],"predecessor-version":[{"id":1050,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/889\/revisions\/1050"}],"up":[{"embeddable":true,"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/pages\/864"}],"wp:attachment":[{"href":"https:\/\/plaza.umin.ac.jp\/wormjp\/wordpress\/wp-json\/wp\/v2\/media?parent=889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}