Jpn. J. Pharmacol. 81 (4), 393 - 396 (1999)

Influence of Chronic Treatment With Imipramine on mRNA Levels in Rat Brain: Elevation of Glyceraldehyde-3-phosphate Dehydrogenase Levels

Michihisa Tohda, Zhao Qi and Hiroshi Watanabe

Department of Pharmacology, Institute for Wakan-yaku, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930 - 0194, Japan

Abstract: The differential display method was used to identify the intrinsic factor that changes its mRNA expression level in rat brain after a 14-day oral administration of 20 mg/kg imipramine. The expression of a 180-bp band was markedly enhanced by imipramine. The results of sequencing and a data base search revealed that the isolated clone was glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with a one-base difference. Enhancement of the expression by imipramine was observed in the amygdala. Quantitative PCR showed that imipramine treatment significantly elevated the GAPDH/â└-actin ratio in the cortex. These findings suggest that long-term treatment with imipramine stimulates GAPDH mRNA expression.

Keywords: Differential display, Antidepressant, Glyceraldehyde-3-phosphate dehydrogenase

Copyrightę The Japanese Pharmacological Society 1999

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