Jpn. J. Pharmacol. 81 (3), 305 - 308 (1999)

Effects of Toluene on Regulation of Adenylyl Cyclase by Stimulation
of G-Protein-Coupled Receptors Expressed in CHO Cells

Hirofumi Tsuga#, Rui-Sheng Wang and Takeshi Honma*

Division of Health Effects Research, National Institute of Industrial Health, Kawasaki, Kanagawa 214 - 8585, Japan
# Domestic research fellowship of Japan Science and Technology Corporation.
*To whom reprint requests should be addressed.

Abstract: We determined the effects of toluene exposure on activation or inhibition of adenylyl cyclase by stimulating human 2-adrenergic receptors (2-AR) and muscarinic acetylcholine receptor (mAChR) m2 subtypes, respectively, expressed in CHO cells. The formation of cAMP via 2-AR stimulation was slightly but not significantly facilitated in the presence of 3.7 M toluene. On the other hand, the inhibition of adenylyl cyclase by 10 M of carbamylcholine stimulation of mAChR m2 subtypes was attenuated in the presence of toluene. These results strongly suggest that toluene affects activation of Gi rather than Gs.

Keywords: G-protein-coupled receptor, Adenylyl cyclase, Toluene

Copyright The Japanese Pharmacological Society 1999

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