Hirofumi Tsuga#, Rui-Sheng Wang and Takeshi Honma*
Division of Health Effects Research, National Institute of Industrial Health, Kawasaki, Kanagawa 214 - 8585, Japan
# Domestic research fellowship of Japan Science and Technology Corporation.
*To whom reprint requests should be addressed.
Abstract: We determined the effects of toluene exposure on activation or inhibition of adenylyl cyclase by stimulating human ƒÀ2-adrenergic receptors (ƒÀ2-AR) and muscarinic acetylcholine receptor (mAChR) m2 subtypes, respectively, expressed in CHO cells. The formation of cAMP via ƒÀ2-AR stimulation was slightly but not significantly facilitated in the presence of 3.7 ƒÊM toluene. On the other hand, the inhibition of adenylyl cyclase by 10 ƒÊM of carbamylcholine stimulation of mAChR m2 subtypes was attenuated in the presence of toluene. These results strongly suggest that toluene affects activation of Gi rather than Gs.
Keywords: G-protein-coupled receptor, Adenylyl cyclase, Toluene