Jpn. J. Pharmacol. 81 (3), 271 - 278 (1999)

Characterization of the Mouse ƒ¿1D-Adrenergic Receptor Gene

Kei Arai1,2, Akito Tanoue2, Nobuhito Goda2, Masayuki Takeda1,
Kota Takahashi1 and Gozoh Tsujimoto2,*

1Department of Urology, Faculty of Medicine, Niigata University, Niigata 951 - 8510, Japan
2Department of Molecular and Cellular Pharmacology, National Children's Medical Research Center, Tokyo 154 - 8509, Japan
(*) To whom correspondence should be addressed.

Abstract: ƒ¿1-Adrenergic receptors (ƒ¿1-ARs) play critical roles in the regulation of a variety of physiological processes. Increasing evidence suggests that multiple receptor subtypes of ƒ¿1-ARs regulate these physiological processes. Molecular cloning has identified three distinct cDNAs encoding ƒ¿1-AR subtypes (ƒ¿1A, ƒ¿1B and ƒ¿1D) that are structurally homologous. Among the ƒ¿1-AR subtypes, the function of the ƒ¿1D-AR remains unclear. In order to examine the physiological role of ƒ¿1D-AR, we cloned and characterized a gene for the mouse ƒ¿1D-AR. Using a mouse ƒ¿1D-AR cDNA as a probe, we isolated the gene for the mouse ƒ¿1D-AR from a mouse genomic library. The ƒ¿1D-AR consists of two exons and an intron that interrupts the coding region of the putative sixth transmembrane domain. The 5'-flanking region of exon 1 contains neither a TATA box nor a CAAT box but is high in GC content and contains several Sp1 binding sites (GC boxes). This pattern is similar to promoters described for other members of ƒ¿1-ARs. The untranslated region also contains putative cyclic AMP response elements. Isolation of this gene will allow further investigation, via gene knock-outs and deletion mutants, of the mechanisms of transcriptional regulation and a greater understanding of the physiological role of ƒ¿1D-AR.

Keywords: ƒ¿1D-Adrenergic receptor, Gene

Copyright© The Japanese Pharmacological Society 1999

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