Kei Arai1,2, Akito Tanoue2, Nobuhito Goda2,
Kota Takahashi1 and Gozoh Tsujimoto2,*
1Department of Urology, Faculty of Medicine, Niigata University, Niigata 951 - 8510, Japan
2Department of Molecular and Cellular Pharmacology, National Children's Medical Research Center, Tokyo 154 - 8509, Japan
(*) To whom correspondence should be addressed.
Abstract: ¿1-Adrenergic receptors (¿1-ARs) play critical roles in the regulation of a variety of physiological processes. Increasing evidence suggests that multiple receptor subtypes of ¿1-ARs regulate these physiological processes. Molecular cloning has identified three distinct cDNAs encoding ¿1-AR subtypes (¿1A, ¿1B and ¿1D) that are structurally homologous. Among the ¿1-AR subtypes, the function of the ¿1D-AR remains unclear. In order to examine the physiological role of ¿1D-AR, we cloned and characterized a gene for the mouse ¿1D-AR. Using a mouse ¿1D-AR cDNA as a probe, we isolated the gene for the mouse ¿1D-AR from a mouse genomic library. The ¿1D-AR consists of two exons and an intron that interrupts the coding region of the putative sixth transmembrane domain. The 5'-flanking region of exon 1 contains neither a TATA box nor a CAAT box but is high in GC content and contains several Sp1 binding sites (GC boxes). This pattern is similar to promoters described for other members of ¿1-ARs. The untranslated region also contains putative cyclic AMP response elements. Isolation of this gene will allow further investigation, via gene knock-outs and deletion mutants, of the mechanisms of transcriptional regulation and a greater understanding of the physiological role of ¿1D-AR.
Keywords: ¿1D-Adrenergic receptor, Gene