Jpn. J. Pharmacol. 80 (4), 375 - 378 (1999)

Both Wortmannin and Simvastatin Inhibit the Adipogenesis in 3T3-L1 Cells During the Late Phase of Differentiation

Koji Tomiyama, Eisuke Nishio and Yasuhiro Watanabe

Department of Pharmacology, National Defense Medical College, 3 - 2 Namiki, Tokorozawa, Saitama 359 - 0042, Japan

Abstract: As we reported previously, both wortmannin and lovastatin inhibit the differentiation in 3T3-L1 cells when these drugs were applied during the insulin-induced cell differentiation. In the present study, 3T3-L1 cells were treated with wortmannin and simvastatin after the completion of the insulin-stimulation, and differentiation was found to be significantly decreased by these drugs. This suggests intracellular signaling pathways play roles in the differentiation of 3T3-L1 cells even after the completion of the insulin-stimulation and also suggests that not only the early phase (day 0 to day 2) but also the late phase (day 2 to day 4) of differentiation is important for the differentiation of the cells.

Keywords: Simvastatin, Wortmannin, Adipocytic differentiation

Copyrightę The Japanese Pharmacological Society 1999

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