Koji Tomiyama, Eisuke Nishio and Yasuhiro Watanabe
Department of Pharmacology, National Defense Medical College, 3 - 2 Namiki, Tokorozawa, Saitama 359 - 0042, Japan
Abstract: As we reported previously, both wortmannin and lovastatin inhibit the differentiation in 3T3-L1 cells when these drugs were applied during the insulin-induced cell differentiation. In the present study, 3T3-L1 cells were treated with wortmannin and simvastatin after the completion of the insulin-stimulation, and differentiation was found to be significantly decreased by these drugs. This suggests intracellular signaling pathways play roles in the differentiation of 3T3-L1 cells even after the completion of the insulin-stimulation and also suggests that not only the early phase (day 0 to day 2) but also the late phase (day 2 to day 4) of differentiation is important for the differentiation of the cells.
Keywords: Simvastatin, Wortmannin, Adipocytic differentiation