Yasuko Sakurai-Yamashita (1), Kohei Takada (1), Keiko Takemura (1), Kimihiro
Yamashita (2), Akihito Enjoji (3), Takashi Kanematsu (3) and Kohtaro Taniyama
Departments of (1) Pharmacology and (3) Surgery II, Nagasaki University School of Medicine, Nagasaki 852-8523, Japan
(2) Section of Pharmacology, Department of Environmental Conservation, Faculty of Environmental Studies, Nagasaki University, Nagasaki 852-8521, Japan
Abstract: Ability of mosapride, a gastrokinetic agent, to bind to 5-HT4 receptor was examined in the stomach of human and guinea pig by in vitro receptor autoradiography. [125I]SB207710 binding sites were detected in the muscle layer including the myenteric plexus of the stomach from both humans and guinea pigs, although the binding was observed more clearly and densely in the stomach of guinea pigs than humans. Mosapride as well as SB204070 inhibited the binding of [125I]SB207710. Thus, mosapride possesses the ability to bind to 5-HT4 receptors of human stomach and may modulate the motility, as in the case of guinea pig stomach.
Keywords: Mosapride, [125I]SB207710 binding, Stomach (human, guinea pig)