Jpn. J. Pharmacol. 79 (3), 387-391 (1999)

Antinociceptive Mechanism of Gosha-jinki-gan in Streptozotocin-Induced Diabetic Animals: Role of Nitric Oxide in the Periphery

Yasuyuki Suzuki (1), Kazuhiro Goto (1), Atsushi Ishige (1), Yasuhiro Komatsu (1) and Junzo Kamei (2)

(1) Kampo and Pharmacognosy Laboratories, Tsumura & Co., Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan
(2) Department of Pathophysiology & Therapeutics, Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan

Abstract: Using streptozotocin-induced diabetic mice and rats, we evaluated the antinociceptive mechanism of Gosha-jinki-gan. The antinociceptive effect of Gosha-jinki-gan (0.3 g/kg, p.o.) in diabetic mice, as determined by the tail-pressure test, was inhibited by NG-nitro-L-arginine methyl ester (L-NAME; 2, 5 mg/kg, i.p.). When L-NAME (10 microg) or methylene blue (500 microg) was topically administered to the intraplantar area of the hind paw, the region used for the paw-pressure test, the antinociceptive activity of Gosha-jinki-gan (0.3 g/kg, p.o.) in diabetic rats was decreased. These results suggested that the antinociceptive effect of Gosha-jinki-gan partly resulted from the peripheral action of increasingly produced nitric oxide.

Keywords: Gosha-jinki-gan, Antinociception, Nitric oxide

Copyrightę The Japanese Pharmacological Society 1999

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