Jpn. J. Pharmacol. 79 (2), 185-193 (1999)

A Decrease in the Amount and Function of Inhibitory GTP-Binding Protein in the Resistance Small Artery From Spontaneously Hypertensive Rats

Motomaru Masutani, Mitsumasa Ohyanagi, Jun Shibuya, Yasuhiro Ishigami and Tadaaki Iwasaki

First Department of Internal Medicine, Hyogo College of Medicine, 1-1, Mukogawa-cho, Nishinomiya 663-8501, Japan

Abstract: The inhibitory GTP-binding protein (Gi protein) plays an important role in regulation of vascular tone. Many studies have implicated the role of Gi protein in conduit vessels. However, the physiological role of Gi protein in the control of peripheral microvascular tone in hypertension has not been established yet. Therefore, we investigated the concentration of Gi protein in the peripheral resistance arteries and aorta in the spontaneously hypertensive rats (SHR), normotensive Wistar-Kyoto rats (WKY) and renovascular hypertensive rats (RHR), using immunohistochemical methods semiquantitatively. Changes in the function of Gi protein in relation to alpha2-adrenoceptor were also investigated by microcannulation techniques. We have shown that the amount of alpha2 subunits of Gi protein in the cremaster small artery was significantly lower in SHR aged 4 weeks and older than in age-matched WKY and that there were no significant differences between RHR and WKY. We also demonstrated that the function of Gi protein in relation to alpha2-adrenoceptor was already lower in SHR before the onset of hypertension. The quantitative and functional decline in Gi protein in the smooth muscle cells of peripheral small arteries were observed in SHR even before the onset of hypertension, whereas rats with secondary hypertension did not exhibit this finding.

Keywords: Gi protein, Spontaneously hypertensive rats, Resistance artery, Immunohistochemistry, alpha2-Adrenoceptor

Copyrightę The Japanese Pharmacological Society 1999

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