Takashi Asada (1), Toshikazu Ogawa (2), Michio Iwai (3) and Masakazu
(1) Molecular Biological Research Laboratories and (2) Pharmacological Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka 532-8514, Japan
(3) Department of Chemistry, Marine Technical College, Ashiya 659-0026, Japan
(4) Fujisawa Research Institute of America, Inc., Northwestern University, Evanston, IL 60201-3135, USA
Abstract: We investigated the effect of recombinant insulin-like growth factor-I (rhIGF-I) on the expression of glucose transporters, GLUT 2 in the liver and GLUT 4 in the heart, in streptozotocin (STZ)-diabetic rats by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. GLUT 2 and its mRNA in the liver was elevated, whereas GLUT 4 and its mRNA in the heart were decreased in STZ-diabetic rats. A two-week treatment with rhIGF-I mostly restored the expression of GLUT 2 and GLUT 4 to normal rat level. We demonstrated that the effect of IGF-I on the expressions of GLUTs was almost the same as that of insulin.
Keywords: Glucose transporter, Reverse transcription-polymerase chain reaction (RT-PCR), Immunohistochemistry