Jpn. J. Pharmacol. 77 (2), 173-176 (1998)

Effects of Transforming Growth Factor Alpha and Hepatocyte Growth Factor on Acid-Induced Damage in Rat Gastric Epithelial Cells

Osamu Furukawa (1), Hiroshi Matsui (2), Noriko Suzuki (1) and Susumu Okabe (1)

(1) Department of Applied Pharmacology, Kyoto Pharmaceutical University, Misasagi, Yamashina, Kyoto 607-8414, Japan
(2) Department of Gastroenterology, Institute of Clinical Medicine, Tsukuba University, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan

Abstract: We examined the effects of transforming growth factor alpha (TGF-alpha) and hepatocyte growth factor (HGF) on acid-induced damage in a rat gastric epithelial cell line. Pretreatment of cells for 30 min with TGF-alpha, but not HGF, significantly prevented the acid-induced cell damage in a concentration-dependent manner. Genistein significantly reduced the protective effect of TGF-alpha. DNA synthesis in RGM1 cells was increased when the cells were incubated with TGF-alpha and HGF for 24 hr. We conclude that the protective effect of TGF-alpha against acid-induced damage seems to be caused by the activation of Na+/H+ exchangers and not by enhanced DNA synthesis.

Keywords: Transforming growth factor alpha, Cell protection, Na+/H+ exchanger

Copyrightę The Japanese Pharmacological Society 1998

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