Jpn. J. Pharmacol. 76 (2), 219-222 (1998)

Protective Effect of MKC-231, a Novel High Affinity Choline Uptake Enhancer, on Glutamate Cytotoxicity in Cultured Cortical Neurons

Akinori Akaike (1), Takehiko Maeda (1), Satoshi Kaneko (1,#) and Yutaka Tamura (2)

(1) Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan
(2) Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama 729-02, Japan
(#) Present address: Yokohama Research Center, Mitsubishi Chemical Corporation, Yokohama 277, Japan

Abstract: This study was performed to examine the neuroprotective action of 2-(2-oxopyrrolidin-1-yl)-N-(2,3-dimethyl-5,6,7,8-tetrahydrofuro
[2,3-b]quinolin-4-yl)acetoamide (MKC-231), which enhances choline uptake and acetylcholine release in the central nervous system. Glutamate neurotoxicity was assessed with cortical cultures obtained from fetal rats. Exposure of cultures to MKC-231 for 12 - 24 hr ameliorated glutamate cytotoxicity. MKC-231 reduced cytotoxicity induced by ionomycin, a calcium ionophore, but did not affect the cytotoxicity induced by S-nitrosocysteine, a nitric oxide (NO) donor. These findings suggest that MKC-231 protects against glutamate neurotoxicity by suppressing the NO formation triggered by Ca2+-influx.

Keywords: Cerebral cortex, Glutamate, MKC-231

Copyrightę The Japanese Pharmacological Society 1998

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