Koji Nishizawa and Akira Yamashita (*)
Kampo (Traditional Chinese Medicine) Research Laboratories, Kanebo, Ltd., 5-90, Tomobuchi-cho 1-chome, Miyakojima-ku, Osaka 534, Japan
(*) To whom correspondence should be addressed.
Abstract: The effects of Kamikihi-To (KMK), a traditional Chinese medicine, on behavioral changes induced by methyl-beta-carboline-3-carboxylate (beta-CCM) were evaluated in mice and rats. beta-CCM, an anxiogenic benzodiazepine receptor inverse agonist (3.0 mg/kg, i.v. administered 1 min before the test), decreased the locomotor activity of mice in a novel environment. Furthermore, beta-CCM (0.1 mg/kg, i.v. administered 10 min before the test) facilitated the suppression of drinking behavior induced by punishment in the water lick conflict test in rats. KMK (1.0 and 2.0 g/kg, p.o. administered 1 hr before the test) antagonized the decreased locomotor activity in the beta-CCM-treated mice. KMK (2.0 g/kg, p.o.) also recovered the suppression of drinking behavior in the beta-CCM-treated rats. KMK (2.0 g/kg, p.o.) had no effect on beta-CCM-untreated mice and rats in these tests. These findings suggest that KMK has a protective effect against beta-CCM-induced behavioral changes.
Keywords: Kamikihi-To, Anxiety, Methyl-beta-carboline-3-carboxylate, Locomotor activity, Water-lick test