Jpn. J. Pharmacol. 73 (4), 347-351 (1997)

Phorbol 12-Myristate 13-Acetate (PMA)-Induced Oxyradical Production in Rheumatoid Synovial Cells

Takatoshi Tanabe (1,2), Hitomi Otani (1), Katsuyuki Mishima (1), Ryokei Ogawa (2) and Chiyoko Inagaki (1,*)

(1) Department of Pharmacology and (2) Department of Orthopaedic Surgery, Kansai Medical University, Moriguchi, Osaka 570, Japan
(*) To whom correspondence should be addressed.

Abstract: We successfully detected the oxyradical production in human synovial A (macrophage-like) and B (fibroblast-like) cells by phorbol 12-myristate 13-acetate (PMA) using the luminol-chemiluminescence method. The PMA (0.1 microg/ml)-induced photon generation was abolished by an O2- scavenger, superoxide dismutase, and an H2O2 scavenger, catalase, suggesting that the stimulus produced oxyradicals in synovial cells. Both of these responses were abolished by a protein kinase C (PKC) inhibitor, calphostine C, but unaffected by an intracellular Ca2+ chelator, BAPTA-AM, and Ca2+ removal from the extracellular medium. These findings suggest that synovial A and B cells produce oxyradicals through PKC-mediated and [Ca2+]i-independent mechanisms, probably through the activation of NADPH oxidase.

Keywords: Synovial cell, Oxyradical production, Protein kinase C

Copyrightę The Japanese Pharmacological Society 1997

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