Jpn. J. Pharmacol. 71 (3), 259-262 (1996)

Affinity Purification of Macrophage Migration Inhibitory Factor/Glycosylation Inhibiting Factor (MIF/GIF) from Bovine Brain by Using a Peptide Ligand Derived from a Novel Serpin

Masahiro Nishibori (1), Naoki Nakaya (1), Shuji Mori (2), Masahiro Kawabata (1), Akihito Tahara (1) and Kiyomi Saeki (1,*)

(1) Department of Pharmacology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700, Japan (2) School of Health Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama 700, Japan (*) To whom correspondence should be addressed.

Abstract: We purified macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using an affinity column with the C-terminal region peptide of a novel serpin as a ligand. The affinity purified preparation showing a single band on SDS-PAGE contained four peptides on RP-HPLC, which were converged into two peptides time-dependently. Sequence analysis and Western blotting revealed that one was identical to bovine MIF/GIF and the other was an N-terminally modified form of MIF/GIF. These results indicated that there exist at least two forms of MIF/GIF in the bovine brain and that they have an affinity for the C-terminal portion of the serpin.

Keywords: Macrophage migration inhibition factor/glycosylation inhibiting factor (MIF/GIF), Brain, Serpin

Copyright© The Japanese Pharmacological Society 1996

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