Jpn. J. Pharmacol. 70 (1), 89-93 (1996)

Intracellular Calcium Oscillation in Cultured Rat Hippocampal Neurons: A Model for Glutamatergic Neurotransmission

Tatsuro Tanaka, Hiroshi Saito and Norio Matsuki (*)

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113, Japan (*) To whom correspondence should be addressed.

Abstract: Neurons can form a synaptic network in culture and show spontaneous oscillation of intracellular Ca2+ concentration ([Ca2+]i). In the present study, spontaneous oscillation of [Ca2+]i was characterized in cultured hippocampal neurons. The oscillation was blocked completely by tetrodotoxin, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and nicardipine, while DL-2-amino-5-phosphonovaleric acid (APV) showed only a partial depression of the increase in [Ca2+]i. These results suggest that the oscillation in [Ca2+]i is mainly mediated by non-N-methyl-D-aspartate (NMDA) type glutamatergic transmission. The oscillation of [Ca2+]i may be a good model for analyzing glutamatergic transmission and synapse formation.

Keywords: Glutamatergic neurotransmission, Synapse formation, non-NMDA receptor

Copyright© The Japanese Pharmacological Society 1996

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