Jpn. J. Pharmacol. 85 (3), 278-281 (2001)

Phosphate Stimulates Differentiation and Mineralization of the Chondroprogenitor Clone ATDC5

Takashi Fujita, Toru Meguro, Nobuo Izumo, Chigusa Yasutomi, Ryo Fukuyama, Hiromichi Nakamuta and Masao Koida*

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Nagaotogecho 45-1, Hirakata 573-0101, Japan
*Corresponding author. FAX: +81-72-866-3108

Abstract: ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3-30 mM) plus ascorbic acid (50 mg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) /PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3-30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis.

Keywords: Phosphate, ATDC5, Endochondral osteogenesis

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