Jpn. J. Pharmacol. 85 (2), 203-206 (2001)

Short Term Hypercholesterolemia Alters NG-Nitro-L-arginine- and Indomethacin-Resistant Endothelium-Dependent Relaxation by Acetylcholine in Rabbit Renal Artery

Hideo Honda1,*, Hiroko Moroe1, Hiroyuki Fujii1, Katsunori Arai2, Yoko Notoya2 and Hiroshi Kogo1

1Department of Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan
2Department of Internal Medicine, Tokyo Medical University, 6-7-1, Nishi-shinjuku, Shinjuku, Tokyo 160-0023, Japan
*Corresponding author. FAX: +81-426-76-4529

Abstract: The tension of isolated rings was measured isometrically to compare the N G-nitro-L-arginine- and indomethacin-resistant relaxation by acetylcholine (ACh) in the renal artery from normal rabbits and short term hypercholesterolemia rabbits (0.5% cholesterol chow for 5 weeks). ACh-induced relaxation in the renal artery precontracted with phenylephrine was not influenced by cholesterol-enriched chow. However, in comparison with artery from normal rabbits, the N G-nitro-L-arginine- and indomethacin-resistant endothelium-dependent relaxation by ACh was significantly enhanced by the chow. The resistant part of ACh-induced relaxation was significantly inhibited when the artery was treated with tetraethylammonium or SKF 525a. Results suggest that short term hypercholesterolemia modulates endothelium-derived hyperpolarizing factor-mediated relaxation in rabbit renal artery.

Keywords: Hypercholesterolemia, Renal artery, Endothelium-derived hyperpolarizing factor

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