Jpn. J. Pharmacol. 84 (3), 355-359 (2000)


Effect of APGW-Amide on [Ca2+]i in Rat Pheochromocytoma PC12 Cells

Xiao Yan Han1, Yuzuru Ito2, Yoshinori Nozawa2, Yifa Jiang3 and Kenichi Matsunami1,*

1Department of Neurophysiology, Institute of Equilibrium Research, and 2Department of Biochemistry,
Gifu University School of Medicine, Gifu 500-8705, Japan
3Virtual System Laboratory, Gifu University, Gifu 501-1193, Japan
*Corresponding author.  FAX:+81-58-267-2954
E-mail: kmm@cc.gifu-u.ac.jp


Abstract: In order to determine whether Ala-Pro-Gly-Try-NH2 (APGW-amide) could affect mammalian excitable cells, we investigated the effect of APGW-amide in PC12 cells. APGW-amide caused a rapid [Ca2+]i elevation, which was completely prevented by elimination of extracellular Ca2+ with EGTA and inhibited by two L-type Ca2+ channel blockers. [Ca2+]i elevation was also blocked by a specific PKC inhibitor and prolonged pretreatment of cells with PMA. These results indicate that APGW-amide elevates [Ca2+]i in PC12 cells, possibly by Ca2+ influx via L-type Ca2+ channel activated by PKC.

Keywords: APGW-amide, PC12 cell, L-type Ca2+ channel


Copyright© The Japanese Pharmacological Society 2000

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