Kazunori Shigesue, Noriko Kodama and Hiroaki Nanba*
Department of Microbial Chemistry, Kobe Pharmaceutical University,
19-1, Motoyama-kitamachi 4-chome, Higashinada-ku, Kobe 658-8558, Japan
Abstract: We recently reported the anti-hepatitis effect of a polysaccharide, designated as the D-fraction, extracted from maitake. Its effect includes immuno-regulating activities. We investigated the effect of the glucan in collagen-induced arthritis (CIA). The D-fraction was administered to CIA mice for 30 consecutive days. Arthritis development was observed from the 4th day after the second immunization. The D-fraction did not have any influence on anti-type╩II collagen antibodies in blood serum or activated B╩cells. To determine how cellular immunity may be involved in the development of CIA, ratios of CD4+ T╩cells and their activated form in the axillary and inguinal lymph node T╩cells were detected by flow cytometry analysis. The ratios were not different between the D-fraction group and the control group. However, interleukin-1b, granulocyte-macrophage colony-stimulating factor and tumor necrosis factor-a productions from splenic macrophages were significantly increased to 2.0, 4.7 and 1.9╩times the control group level, respectively. The ratio of macrophages in the whole spleen cells was 2.3╩times that of the control group, and their migrating ability was 1.9╩times higher. Based on these results, we concluded that the arthritis development induced by D-fraction administration is attributable to the activation of splenic macrophages.
Keywords: Collagen-induced arthritis, Maitake polysaccharide, Splenic macrophage
Copyrightę The Japanese Pharmacological Society 2000
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