Jpn. J. Pharmacol. 84 (2), 206-212 (2000)


Recombinant Adeno-associated Virus Vectors Efficiently Transduce Foreign Gene Into Bovine Aortic Endothelial Cells: Comparison With Adenovirus Vectors

Shinji Teramoto1*, Takeo Ishii2, Takeshi Matsuse3 and Yoshinosuke Fukuchi4

1Department of Internal Medicine, San-no Hospital, Tokyo (Medical Research Center, International University of Health and Welfare), 8-5-35 Akasaka, Minato-ku Tokyo 107-0052, Japan
2Department of Geriatric Medicine, Tokyo University Hospital, Tokyo 113-8655, Japan
3Department of Pulmonary Medicine, Yokohama City University Medical Center, Yokohama 232-0024, Japan
4Department of Respiratory Medicine, Juntendo University School of Medicine, Tokyo 113-8421, Japan
*Corresponding author.╩╩FAX:+81-3-3404-3652
E-mail: shinjit-tky@umin.ac.jp


Abstract: Because the features and kinetics of adeno-associated virus (AAV)-mediated gene transfer to endothelial cells (EC) are yet to be ultimately determined, we tested variables pertinent to the efficiency of AAV-mediated gene transfer to bovine aortic endothelial cells (BAEC). The variables with AAV vectors were compared with the better characterized adenovirus (Ad) vectors. There is a dose-response relationship between multiplicity of infection (moi) of AAV or Ad vectors and transduction efficiency in BAEC. The higher moi of AAV vectors achieved more than 80% of transduction efficiency in cultured BAEC. AAV and Ad vectors showed an incubation-time-dependent increase in transduction efficiency of LacZ gene to the BAEC up to 12╩h of vector exposure. Although the similar kinetics of transduction efficiency of LacZ gene to BAEC was found in both vectors, the duration of gene expression was longer in AAV vector than that in Ad vectors in vitro. These results indicate that AAV-vector is efficient for gene transfer to EC, and higher moi of vectors or a longer period exposure of vectors to EC can facilitate efficient transduction of a foreign gene into cultured EC. For the duration of gene expression, the AAV vectors may be better than Ad vectors.

Keywords: Endothelial cell, Gene transfer, Adeno-associated virus vector, Adenovirus vector


Copyrightę The Japanese Pharmacological Society 2000

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