Jpn. J. Pharmacol. 84 (1), 71-74 (2000)

Serotonin Induces Apoptosis in PGT-b Pineal Gland Tumor Cells

Sung-Vin Yim1, Kyung-Hwan Kim2, Chang-Ju Kim3 and Joo-Ho Chung4,*

1DepartmentÊof Pharmacology, College of Medicine, Kangwon National University, Hyoja-Dong, Chuncheon, Kangwon-Do, 200-701, Korea
2InjeÊUniversity, Sanggye-Baek Hospital, Seoul 139-707, Korea
Departments of 3Physiology and 4Pharmacology, College of Medicine, Kohwang Medical Research Institute, Kyung Hee University,
1 Hoegi-Dong, Tongdaemoon-Ku, Seoul 130-701, Korea
*ÊTo whom correspondence should be addressed.

Abstract: Serotonin-induced neuronal cell death has been implicated as a possible cause of neurodegenerative and neuropsychiatric disorders. To investigate the involvement of serotonin-induced apoptosis as a potential mechanism in the pathophysiology of serotonin-related diseases, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI (4¢,6-diamidino-2-phenylindole) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) assay and flow cytometric analysis were performed using the immortalized pineal cell line PGT-b. Through morphological and biochemical analyses, it was demonstrated that cell death induced by serotonin in PGT-b cells shows classic apoptotic features. These data suggest that serotonin induces apoptosis in PGT-b cells.

Keywords: Serotonin, Cytotoxicity, Apoptosis

Copyright© The Japanese Pharmacological Society 2000

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