Jpn. J. Pharmacol. 82 (1), 78-81 (2000)

Peroxynitrite–Generating Species: Good Candidate Oxidants
in Aqueous Extracts of Cigarette Smoke

Yu Yamaguchi1, Satomi Kagota1, Jun Haginaka2 and Masaru Kunitomo1

1Department of Pharmacology and 2Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences,
Mukogawa Women's University, 11–68, Koshien Kyuban–cho, Nishinomiya 663–8179, Japan

Abstract: Cigarette smoking is a well–known risk factor for atherosclerosis, but the mechanism of the adverse biological effect of smoking remains to be established. Cigarette smoke contains high concentrations of free radicals and oxidants. We show here that cigarette smoke extracts (CSE), prepared by bubbling the gas phase of smoke into phosphate–buffered saline, could convert tyrosine to 3–nitrotyrosine. The tyrosine nitration terminated 6 h after incubating tyrosine with CSE at 37°C. These results indicate that the active oxidants in CSE are peroxynitrite–generating species like 3–morpholinosydnonimine (SIN–1), suggesting that they modify plasma lipoproteins and contribute to the pathogenesis of atherosclerosis.

Keywords: Cigarette smoke, Peroxynitrite, 3–Morpholinosydnonimine (SIN–1)

Copyright© The Japanese Pharmacological Society 2000

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