Jpn. J. Pharmacol. 82 (1), 29-33 (2000)

Anti–type I Allergic Mechanisms of Mao–bushi–saishin–to in Mice

Yoshiki Ikeda1, Akira Iizuka1, Sakae Amagaya1, Atsushi Ishige1, Yasuhiro Komatsu1 and Eiichi Fujihira2

1CentralResearch Laboratories, Tsumura & Co., 3586, Yoshiwara, Ami–machi, Inashiki–gun, Ibaraki 300–1192, Japan
2Departmentof Pharmacotherapy, Hokkaido College of Pharmacy, 7–1, Katsuraoka–machi, Otaru, Hokkaido 047–0264, Japan

Abstract: We investigated the anti–allergic effect of mao–bushi–saishin–to (MBS) on the typeI allergy model in mice. When MBS was administered orally at a dose of 0.5 or 1.0 g/kg, edema of the footpad, the amount of plasma IgE and the ratio of eosinophilic leukocytes in peritoneal exudate cells were all dose–relatedly suppressed. Moreover to investigate the anti–typeI allergic mechanisms of MBS, enzyme–linked immunosorbent assay was performed to determine the interleukin (IL)–4, IL–5 and interferon (IFN)–gamma production from splenocytes that were stimulated by pokeweed mitogen for 48 h. In addition, we assayed IgE production from splenic Bcells stimulated with the lipopolysaccharide and IL–4 for 7 days. MBS inhibited the IL–4 and IFN–gamma production, but IL–5 and IgE production were not affected. Thus possibly, the inhibition of IL–4 production may partially be involved in the expression of the anti–typeI allergic effects of MBS.

Keywords: Mao–bushi–saishin–to, Anti–allergy, Interleukin–4, IgE, Spleen cell

Copyright© The Japanese Pharmacological Society 2000

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